Anti-HDAC6 antibody - C-terminal (ab196351)

Overview

  • Product name
    Anti-HDAC6 antibody - C-terminal
    See all HDAC6 primary antibodies
  • Description
    Goat polyclonal to HDAC6 - C-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Chimpanzee, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human HDAC6 aa 1202-1215 (C terminal) (Cysteine residue). (NP_006035.2).
    Sequence:

    C-AHQNKFGEDMPHPH


    Database link: Q9UBN7

  • Positive control
    • HeLa nuclear lysate; Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab196351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 131 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Images

  • Anti-HDAC6 antibody - C-terminal (ab196351) at 1 µg/ml + HeLa nuclear lysate at 35 µg

    Predicted band size: 131 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue, labeling HDAC6 with ab196351 at 5 µg/ml.

References

ab196351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (brain)
Permeabilization
Yes - 0.5% Triton-100
Specification
brain
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
None
Username

Zhongjie Shi

Verified customer

Submitted Aug 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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