Recombinant Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1698(2)] to HDAC6
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-HDAC6 antibody [EPR1698(2)]
See all HDAC6 primary antibodies -
Description
Rabbit monoclonal [EPR1698(2)] to HDAC6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Monkey -
Immunogen
Synthetic peptide within Human HDAC6 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UBN7 -
Positive control
- WB: HAP1, HeLa, Jurkat, and COS-1 cell lysates. Flow Cyt (intra): K562. IHC-P: Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1698(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/200.
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/200. |
WB
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
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Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
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All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HDAC6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab133493 was shown to react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264804 (knockout cell lysate ab257145) was used. Wild-type HeLa and HDAC6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] (ab133493)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HDAC6 with ab133493 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Lanes 1-2 : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lanes 3-4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution
Lanes 1 & 3 & 5 : Wild-type HAP1 cell lysate at 20 µg
Lanes 2 & 4 : HDAC6 knockout HAP1 cell lysate at 20 µg
Lane 6 : HDAC6 knockout HAP1 cell lysate
Predicted band size: 131 kDaLanes 1 and 2: Green signal from target – ab133493 observed at 160 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signalab133493 was shown to specifically react with HDAC6 when HDAC6 knockout samples were used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. ab133493 and ab8226 (loading control to beta actin) were diluted 1/10 000 and 1/1000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : COS-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab133493 has been referenced in 6 publications.
- Zheng Y et al. HDAC6, modulated by miR-206, promotes endometrial cancer progression through the PTEN/AKT/mTOR pathway. Sci Rep 10:3576 (2020). PubMed: 32107418
- Yang P et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell 181:325-345.e28 (2020). PubMed: 32302571
- Catanese A et al. Retinoic acid worsens ATG10-dependent autophagy impairment in TBK1-mutant hiPSC-derived motoneurons through SQSTM1/p62 accumulation. Autophagy 15:1719-1737 (2019). PubMed: 30939964
- Wattin M et al. Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients. Int J Mol Sci 19:N/A (2018). PubMed: 29316663
- Phadwal K et al. Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates. Sci Rep 8:10004 (2018). PubMed: 29968775
- Li A et al. Histone deacetylase 6 regulates the immunosuppressive properties of cancer-associated fibroblasts in breast cancer through the STAT3-COX2-dependent pathway. Oncogene N/A:N/A (2018). PubMed: 29980788