Product nameAnti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free
See all HDAC6 primary antibodies
DescriptionRabbit monoclonal [EPR1698(2)] to HDAC6 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human, Monkey
Synthetic peptide within Human HDAC6 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UBN7
- HeLa, Jurkat, K562, and COS-1 cell lysates, Human kidney tissue.
Ab210472 is the carrier-free version of ab133493. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab210472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab210472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 160 kDa (predicted molecular weight: 131 kDa).|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133493).
ab210472 has not yet been referenced specifically in any publications.