Overview

  • Product name

    Anti-HDAC6 antibody [EPR22951-29]
    See all HDAC6 primary antibodies
  • Description

    Rabbit monoclonal [EPR22951-29] to HDAC6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse HDAC6 aa 1100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Z2V5

  • Positive control

    • WB: Mouse and rat brain lysate. NIH/3T3, Neuro-2a and PC-12 lysates. FC: Neuro-2a and NIH/3T3 cells. IP: Mouse brain lysate and NIH/3T3 lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 131 kDa.
Flow Cyt 1/600.
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
      Contains 1 UBP-type zinc finger.
    • Post-translational
      modifications

      Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
      Sumoylated in vitro.
    • Cellular localization

      Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • CPBHM antibody
      • FLJ16239 antibody
      • HD 6 antibody
      • HD6 antibody
      • HDAC 6 antibody
      • HDAC6 antibody
      • HDAC6_HUMAN antibody
      • Histone deacetylase 6 (HD6) antibody
      • Histone deacetylase 6 antibody
      • JM 21 antibody
      • JM21 antibody
      • KIAA0901 antibody
      • OTTHUMP00000032398 antibody
      • OTTHUMP00000197663 antibody
      • PPP1R90 antibody
      • Protein phosphatase 1 regulatory subunit 90 antibody
      see all

    Images

    • All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 131 kDa
      Observed band size: 130 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026). 

      Exposure time: Lane 1-2: 32 seconds Lane 3: 3 minutes.

    • All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 131 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Kidney is a low-expression tissue.

      A positive unidentified band between 15-20 kDa is detected in mouse kidney lysate.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

      Exposure time: 48 seconds.

    • HDAC6 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10µg

      Lane 2: ab239362 IP in NIH/3T3 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in NIH/3T3 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

       

    • HDAC6 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse brain lysate 10µg

      Lane 2: ab239362 IP in mouse brain lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in mouse brain lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 mins.

       

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    References

    ab239362 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239362.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up