Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6159] to HDAC6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-HDAC6 antibody [EPR6159]
See all HDAC6 primary antibodies
DescriptionRabbit monoclonal [EPR6159] to HDAC6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human HDAC6 (UniProt ID: Q9UBN7).
- 293T, HUVEC, HeLa and MCF7 cell lysates; Human papillary carcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 2.65 x 10 -10 M Learn more about KD
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 131 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
All lanes : Anti-HDAC6 antibody [EPR6159] (ab133541) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa
Immunohistochemical analysis of paraffin embedded Human papillary carcinoma tissue labelling HDAC6 using ab133541 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133541 has not yet been referenced specifically in any publications.