Recombinant
RabMAb

Recombinant Anti-HDAC6 antibody [EPR6160] (ab133539)

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Overview

  • Product name

    Anti-HDAC6 antibody [EPR6160]
    See all HDAC6 primary antibodies

  • Description

    Rabbit monoclonal [EPR6160] to HDAC6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HDAC6 aa 1000-1100. The exact sequence is proprietary.

  • Positive control

    • 293T, HepG2, and K562 cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 131 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
      Contains 1 UBP-type zinc finger.

    • Post-translational
      modifications

      Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
      Sumoylated in vitro.

    • Cellular localization

      Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
    • Information by UniProt

    • Database links

      • Alternative names

        • CPBHM antibody
        • FLJ16239 antibody
        • HD 6 antibody
        • HD6 antibody
        • HDAC 6 antibody
        • HDAC6 antibody
        • HDAC6_HUMAN antibody
        • Histone deacetylase 6 (HD6) antibody
        • Histone deacetylase 6 antibody
        • JM 21 antibody
        • JM21 antibody
        • KIAA0901 antibody
        • OTTHUMP00000032398 antibody
        • OTTHUMP00000197663 antibody
        • PPP1R90 antibody
        • Protein phosphatase 1 regulatory subunit 90 antibody
        see all

      Images

      • Western blot - Anti-HDAC6 antibody [EPR6160] (ab133539)
        Western blot - Anti-HDAC6 antibody [EPR6160] (ab133539)
        All lanes : Anti-HDAC6 antibody [EPR6160] (ab133539) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 131 kDa

      • Other - Anti-HDAC6 antibody [EPR6160] (ab133539)
        Other - Anti-HDAC6 antibody [EPR6160] (ab133539)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Yin Z  et al. Overexpression of HDAC6 suppresses tumor cell proliferation and metastasis by inhibition of the canonical Wnt/ß-catenin signaling pathway in hepatocellular carcinoma. Oncol Lett 16:7082-7090 (2018). Read more (PubMed: 30546442) »
      • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). WB ; Human . Read more (PubMed: 22347520) »
      See all 2 Publications for this product

      Publishing research using ab133539? Please let us know so that we can cite the reference in this datasheet.

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