For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    hdac6-antibody-epr6160-ab133539.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
Share by email
RecombinantRabMAb

Recombinant Anti-HDAC6 antibody [EPR6160] (ab133539)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-HDAC6 antibody [EPR6160] (ab133539)
  • OI-RD Scanning - Anti-HDAC6 antibody [EPR6160] (ab133539)
  • Anti-HDAC6 antibody [EPR6160] (ab133539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6160] to HDAC6
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human HDAC6 protein (Active) (ab42632)

View more associated products

Overview

  • Product name

    Anti-HDAC6 antibody [EPR6160]
    See all HDAC6 primary antibodies
  • Description

    Rabbit monoclonal [EPR6160] to HDAC6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human HDAC6 aa 1000-1100. The exact sequence is proprietary.

  • Positive control

    • 293T, HepG2, and K562 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Dissociation constant (KD)

    KD = 5.31 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6160
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class

Associated products

  • Alternative Versions

    • Anti-HDAC6 antibody [EPR6160] - BSA and Azide free (ab248542)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
    • K-562 nuclear extract lysate (ab14851)
  • Recombinant Protein

    • Recombinant human HDAC6 protein (Active) (ab42632)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 131 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 131 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
      Contains 1 UBP-type zinc finger.
    • Post-translational
      modifications

      Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
      Sumoylated in vitro.
    • Cellular localization

      Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
    • Target information above from: UniProt accession Q9UBN7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10013 Human
      • Omim: 300272 Human
      • SwissProt: Q9UBN7 Human
      • Unigene: 6764 Human
      • Alternative names

        • CPBHM antibody
        • FLJ16239 antibody
        • HD 6 antibody
        • HD6 antibody
        • HDAC 6 antibody
        • HDAC6 antibody
        • HDAC6_HUMAN antibody
        • Histone deacetylase 6 (HD6) antibody
        • Histone deacetylase 6 antibody
        • JM 21 antibody
        • JM21 antibody
        • KIAA0901 antibody
        • OTTHUMP00000032398 antibody
        • OTTHUMP00000197663 antibody
        • PPP1R90 antibody
        • Protein phosphatase 1 regulatory subunit 90 antibody
        see all

      Images

      • Western blot - Anti-HDAC6 antibody [EPR6160] (ab133539)
        Western blot - Anti-HDAC6 antibody [EPR6160] (ab133539)
        All lanes : Anti-HDAC6 antibody [EPR6160] (ab133539) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 131 kDa

      • OI-RD Scanning - Anti-HDAC6 antibody [EPR6160] (ab133539)
        OI-RD Scanning - Anti-HDAC6 antibody [EPR6160] (ab133539)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-HDAC6 antibody [EPR6160] (ab133539)
        Anti-HDAC6 antibody [EPR6160] (ab133539)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab133539? Please let us know so that we can cite the reference in this datasheet.

      ab133539 has been referenced in 2 publications.

      • Yin Z  et al. Overexpression of HDAC6 suppresses tumor cell proliferation and metastasis by inhibition of the canonical Wnt/ß-catenin signaling pathway in hepatocellular carcinoma. Oncol Lett 16:7082-7090 (2018). PubMed: 30546442
      • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). WB ; Human . PubMed: 22347520

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab133539.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.