Product nameAnti-HDAC6 antibody - N-terminal
See all HDAC6 primary antibodies
DescriptionRabbit polyclonal to HDAC6 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Synthetic peptide within Human HDAC6 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q9UBN7
- ICC/IF: HeLa cells. WB: HDAC6 transfected HEK-293T whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 131 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
- Information by UniProt
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HDAC6 (green) using ab226959 at 1/500 dilution in ICC/IF. Counterstain: alpha-tubulin filaments were labeled with an anti-alpha Tubulin antibody (red) at 1/2000.
All lanes : Anti-HDAC6 antibody - N-terminal (ab226959) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HDAC6 (3xFlag-tag), whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 131 kDa
5% SDS-PAGE gel.
ab226959 has not yet been referenced specifically in any publications.