Overview

  • Product name

    Anti-HDAC6 antibody - N-terminal
    See all HDAC6 primary antibodies
  • Description

    Rabbit polyclonal to HDAC6 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide within Human HDAC6 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q9UBN7

  • Positive control

    • ICC/IF: HeLa cells. WB: HDAC6 transfected HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 131 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization

    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

Images

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HDAC6 (green) using ab226959 at 1/500 dilution in ICC/IF. Counterstain: alpha-tubulin filaments were labeled with an anti-alpha Tubulin antibody (red) at 1/2000.

  • All lanes : Anti-HDAC6 antibody - N-terminal (ab226959) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HDAC6 (3xFlag-tag), whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 131 kDa



    5% SDS-PAGE gel.

References

ab226959 has not yet been referenced specifically in any publications.

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