Western blot abreview for Anti-HDAC6 (phospho S22) antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (preadipocyte)
Loading amount
4 µg
Specification
preadipocyte
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 0.25%Gelatin in 1XTBST

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 65 kDa

Additional data

Additional Notes
lane1:3T3 L1 nonconfluence 1d
lane2:3T3 L1 nonconfluence 2d
lane3:3T3 L1 nonconfluence 3d
lane4:3T3 L1 confluence 1d
lane5:3T3 L1 confluence 2d
lane6:3T3 L1 confluence 3d

The band near the 62kD marker is the main band detected by
HDAC6(pS22)antibody.But the MW of HDAC6 is about 134kD,and there is no
obvious band detected between 175kD and 83kD . However,what is surprising
is that the pHDAC6 level in the six lanes given by the main band is
consistent with our speculation.So we are looking forward to your reply as
soon as possible. Thanks!
Abcam response
Thank you for your honest feedback. This antibody has not been previously tested in western blotting. It may be that ab61058 does not work in this application. Alternatively, there may be undetectable levels of HDAC6 (phospho S22) in the samples.

Mr. Hongzhong Wang

Verified customer

Submitted Feb 23 2009

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