The band near the 62kD marker is the main band detected by HDAC6(pS22)antibody.But the MW of HDAC6 is about 134kD,and there is no obvious band detected between 175kD and 83kD . However,what is surprising is that the pHDAC6 level in the six lanes given by the main band is consistent with our speculation.So we are looking forward to your reply as soon as possible. Thanks!
Thank you for your honest feedback. This antibody has not been previously tested in western blotting. It may be that ab61058 does not work in this application. Alternatively, there may be undetectable levels of HDAC6 (phospho S22) in the samples.
Mr. Hongzhong Wang
Submitted Feb 23 2009
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