Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HDAC6 (phospho S22) antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Specification
Rat prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in 0.1% PBST
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Additional Notes
HDAC6 was detected in both cytoplasm and nucleus. White arrow indicates cells with positive nuclear staining.

Dr. Armen Petrosyan

Verified customer

Submitted Jun 29 2017

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