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    hdac7-antibody-ab12174.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC7 antibody (ab12174)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (13)

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Western blot - Anti-HDAC7 antibody (ab12174)
  • Immunoprecipitation - Anti-HDAC7 antibody (ab12174)

Key features and details

  • Rabbit polyclonal to HDAC7
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-HDAC7 antibody [EPR10922] (ab166911)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-HDAC7 antibody
    See all HDAC7 primary antibodies
  • Description

    Rabbit polyclonal to HDAC7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KPRLRQIPSAEDLETDG

    conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa and NIH3T3 cell lysates IP: HEK-293T cell lysates
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HDAC7 protein (ab101660)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.

This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide.

WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
IP
Use at an assay dependent concentration.

1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7.

Notes
ICC/IF
Use at an assay dependent concentration.

This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide.

WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
IP
Use at an assay dependent concentration.

1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localization

    Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Target information above from: UniProt accession Q8WUI4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51564 Human
    • Entrez Gene: 56233 Mouse
    • Entrez Gene: 84582 Rat
    • Omim: 606542 Human
    • SwissProt: Q8WUI4 Human
    • SwissProt: Q8C2B3 Mouse
    • SwissProt: Q99P96 Rat
    • Unigene: 200063 Human
    • Unigene: 384027 Mouse
    • Unigene: 203327 Rat
    see all
  • Alternative names

    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Images

  • Western blot - Anti-HDAC7 antibody (ab12174)
    Western blot - Anti-HDAC7 antibody (ab12174)
    All lanes : Anti-HDAC7 antibody (ab12174) at 1/500 dilution

    Lane 1 : HeLa nuclear lysate
    Lane 2 : NIH3T3 cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Predicted band size: 103 kDa

  • Immunoprecipitation - Anti-HDAC7 antibody (ab12174)
    Immunoprecipitation - Anti-HDAC7 antibody (ab12174)

    HDAC7 was immunoprecipitated from whole cell extracts of human HEK-293T cells expressing HDAC7-FLAG using ab12174. The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® and developed using Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate.

    Lane 1: 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of HEK-293T cells expressing HDAC7-FLAG
    Lane 2: Negative control (extract without antibody).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab12174? Please let us know so that we can cite the reference in this datasheet.

ab12174 has been referenced in 13 publications.

  • Sato T  et al. A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction. Nat Commun 11:3282 (2020). PubMed: 32612176
  • Luo Z  et al. Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming. Cell Regen (Lond) 8:21-29 (2019). PubMed: 31205685
  • Caslini C  et al. HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells. Oncogene 38:6599-6614 (2019). PubMed: 31375747
  • Wang C  et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992
  • Anglès F  et al. HDAC inhibitors rescue multiple disease-causing CFTR variants. Hum Mol Genet 28:1982-2000 (2019). PubMed: 30753450
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HDAC7 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat and LNCaP)
Gel Running Conditions
Reduced Denaturing (4-15% TGX BioRad)
Loading amount
200000 cells
Specification
Jurkat and LNCaP
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 05 2016

Western blot abreview for Anti-HDAC7 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Loading amount
20 µg
Specification
liver cancer
Gel Running Conditions
Reduced Denaturing (10%gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 17 2012

Western blot abreview for Anti-HDAC7 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
40 µg
Specification
HCT116 colon cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Read More

Abcam user community

Verified customer

Submitted Nov 07 2006

Western blot abreview for Anti-HDAC7 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted Aug 26 2005

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