Anti-HDAC7 antibody (ab12174)

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Overview

  • Product name

    Anti-HDAC7 antibody
    See all HDAC7 primary antibodies

  • Description

    Rabbit polyclonal to HDAC7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide:

    KPRLRQIPSAEDLETDG

    conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Mouse NIH-3T3 cells (Western blotting) Normal rat kidney (NRK) cells (indirect immunofluorescence)

  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab12174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 105 kDa. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide.
IP Use at an assay dependent dilution. 1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7.
ICC/IF Use at an assay dependent dilution.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.

  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.

  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

  • Post-translational
    modifications

    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.

  • Cellular localization

    Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

References

This product has been referenced in:

  • Witt AE  et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36:1707-1720 (2017). WB, IF . Read more (PubMed: 27694895) »
  • Stemig M  et al. Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF. PLoS One 10:e0123843 (2015). WB ; Mouse . Read more (PubMed: 25875108) »
See all 8 Publications for this product

Publishing research using ab12174? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HDAC7 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat and LNCaP)
Gel Running Conditions
Reduced Denaturing (4-15% TGX BioRad)
Loading amount
200000 cells
Specification
Jurkat and LNCaP
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 05 2016

Western blot abreview for Anti-HDAC7 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Loading amount
20 µg
Specification
liver cancer
Gel Running Conditions
Reduced Denaturing (10%gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 17 2012

Western blot abreview for Anti-HDAC7 antibody

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
40 µg
Specification
HCT116 colon cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Read More

Abcam user community

Verified customer

Submitted Nov 07 2006

Western blot abreview for Anti-HDAC7 antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted Aug 26 2005

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