Anti-HDAC7 antibody (ab12174)
Key features and details
- Rabbit polyclonal to HDAC7
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-HDAC7 antibody
See all HDAC7 primary antibodies -
Description
Rabbit polyclonal to HDAC7 -
Host species
Rabbit -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
KPRLRQIPSAEDLETDG
conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. -
Positive control
- Mouse NIH-3T3 cells (Western blotting) Normal rat kidney (NRK) cells (indirect immunofluorescence)
-
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localization
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
-
Database links
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
see all -
Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
References
ab12174 has been referenced in 11 publications.
- Luo Z et al. Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming. Cell Regen (Lond) 8:21-29 (2019). PubMed: 31205685
- Caslini C et al. HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells. Oncogene 38:6599-6614 (2019). PubMed: 31375747
- Wang C et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992
- Witt AE et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36:1707-1720 (2017). WB, IF . PubMed: 27694895
- Stemig M et al. Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF. PLoS One 10:e0123843 (2015). WB ; Mouse . PubMed: 25875108
