Anti-HDAC7 antibody (ab12174)
Key features and details
- Rabbit polyclonal to HDAC7
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-HDAC7 antibody
See all HDAC7 primary antibodies -
Description
Rabbit polyclonal to HDAC7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
KPRLRQIPSAEDLETDG
conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. -
Positive control
- WB: HeLa and NIH3T3 cell lysates IP: HEK-293T cell lysates
-
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. |
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
|
| IP |
Use at an assay dependent concentration.
1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7. |
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa. |
|
IP
Use at an assay dependent concentration. 1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7. |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localization
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
-
Database links
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
see all -
Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
Images
-
Western blot - Anti-HDAC7 antibody (ab12174)All lanes : Anti-HDAC7 antibody (ab12174) at 1/500 dilution
Lane 1 : HeLa nuclear lysate
Lane 2 : NIH3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 103 kDa -
Immunoprecipitation - Anti-HDAC7 antibody (ab12174)HDAC7 was immunoprecipitated from whole cell extracts of human HEK-293T cells expressing HDAC7-FLAG using ab12174. The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® and developed using Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate.
Lane 1: 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of HEK-293T cells expressing HDAC7-FLAG
Lane 2: Negative control (extract without antibody).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab12174 has been referenced in 13 publications.
- Sato T et al. A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction. Nat Commun 11:3282 (2020). PubMed: 32612176
- Luo Z et al. Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming. Cell Regen (Lond) 8:21-29 (2019). PubMed: 31205685
- Caslini C et al. HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells. Oncogene 38:6599-6614 (2019). PubMed: 31375747
- Wang C et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992
- Anglès F et al. HDAC inhibitors rescue multiple disease-causing CFTR variants. Hum Mol Genet 28:1982-2000 (2019). PubMed: 30753450