Anti-HDAC7 antibody (ab12174)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-HDAC7 antibody
See all HDAC7 primary antibodies -
DescriptionRabbit polyclonal to HDAC7
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
KPRLRQIPSAEDLETDG
conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. -
Positive control
- Mouse NIH-3T3 cells (Western blotting) Normal rat kidney (NRK) cells (indirect immunofluorescence)
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General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody is affinity-purified using the immunizing peptide immobilized on agarose.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab12174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 105 kDa. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. | |
| IP | Use at an assay dependent dilution. 1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7. | |
| ICC/IF | Use at an assay dependent dilution. |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
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DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
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Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
- Information by UniProt
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Database links
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
see all -
Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
Protocols
References
This product has been referenced in:
- Witt AE et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36:1707-1720 (2017). WB, IF . Read more (PubMed: 27694895) »
- Stemig M et al. Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF. PLoS One 10:e0123843 (2015). WB ; Mouse . Read more (PubMed: 25875108) »
