Product nameAnti-HDAC7 antibody
See all HDAC7 primary antibodies
DescriptionRabbit polyclonal to HDAC7
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human HDAC7 aa 902-952. The exact sequence is proprietary. NP_056216.1
Database link: Q8WUI4
- WB: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue; mouse colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245524 was affinity purified using an epitope specific to HDAC7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 103 kDa.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Cellular localizationNucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
- Information by UniProt
- DKFZP586J0917 antibody
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- HD 7a antibody
All lanes : Anti-HDAC7 antibody (ab245524) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Exposure time: 1 minute
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue (left) and mouse colon carcinoma tissue (right) stained for HDAC7 using ab245524.
ab245524 was used at either 1/5000 dilution (left) or 1/1000 dilution (right).
ab245524 has not yet been referenced specifically in any publications.