Overview

  • Product name

  • Description

    Rabbit polyclonal to HDAC7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human HDAC7 aa 902-952. The exact sequence is proprietary. NP_056216.1
    Database link: Q8WUI4

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue; mouse colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Domain

      The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
    • Post-translational
      modifications

      May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    • Cellular localization

      Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZP586J0917 antibody
      • FLJ99588 antibody
      • HD 7a antibody
      • HD7 antibody
      • HD7a antibody
      • HDAC 7 antibody
      • HDAC 7A antibody
      • Hdac7 antibody
      • HDAC7_HUMAN antibody
      • HDAC7A antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7A antibody
      • OTTHUMP00000202813 antibody
      • OTTHUMP00000202814 antibody
      see all

    Images

    • All lanes : Anti-HDAC7 antibody (ab245524) at 0.4 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 103 kDa


      Exposure time: 1 minute
    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue (left) and mouse colon carcinoma tissue (right) stained for HDAC7 using ab245524. 

      ab245524 was used at either 1/5000 dilution (left) or 1/1000 dilution (right).

    References

    ab245524 has not yet been referenced specifically in any publications.

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