Overview

  • Product name

    Anti-HDAC7 antibody - C-terminal
    See all HDAC7 primary antibodies
  • Description

    Rabbit polyclonal to HDAC7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 737-952 of Human HDAC7 (Uniprot ID: Q8WUI4).

  • Positive control

    • HepG2 whole cell lysate; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • Cellular localization

    Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Images

  • Anti-HDAC7 antibody - C-terminal (ab137366) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 103 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling HDAC7 with ab137366 at 1/250 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC7 (green) with ab137366 at 1/1000 dilution. The lower image was co-stained with Hoechst 33342 (blue).

References

ab137366 has not yet been referenced specifically in any publications.

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