Anti-HDAC7 antibody - C-terminal (ab137366)
Key features and details
- Rabbit polyclonal to HDAC7 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC7 antibody - C-terminal
See all HDAC7 primary antibodies -
Description
Rabbit polyclonal to HDAC7 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 737-952 of Human HDAC7 (Uniprot ID: Q8WUI4).
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Positive control
- HepG2 whole cell lysate; HeLa cells; Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 103 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 103 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localization
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 509843 Cow
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- Unigene: 200063 Human
- Unigene: 384027 Mouse
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Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
Images
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Western blot - Anti-HDAC7 antibody - C-terminal (ab137366)Anti-HDAC7 antibody - C-terminal (ab137366) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 103 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC7 antibody - C-terminal (ab137366)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling HDAC7 with ab137366 at 1/250 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-HDAC7 antibody - C-terminal (ab137366)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC7 (green) with ab137366 at 1/1000 dilution. The lower image was co-stained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137366 has been referenced in 1 publication.
- Jing X et al. HDAC7 Ubiquitination by the E3 Ligase CBX4 Is Involved in Contextual Fear Conditioning Memory Formation. J Neurosci 37:3848-3863 (2017). PubMed: 28283560