Anti-HDAC7 antibody [HDAC7-97] (ab50212)
Key features and details
- Mouse monoclonal [HDAC7-97] to HDAC7
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-HDAC7 antibody [HDAC7-97]
See all HDAC7 primary antibodies -
Description
Mouse monoclonal [HDAC7-97] to HDAC7 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide:
YRTLEPLDTEGAARSVLSS
conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7 -
Positive control
- WB: human embryonal kidney 293T cells expressing recombinant human HDAC7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purification notes
Ab50212 is a purified IgG. -
Clonality
Monoclonal -
Clone number
HDAC7-97 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab50212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localization
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
see all -
Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
Images
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![Western blot - Anti-HDAC7 antibody [HDAC7-97] (ab50212)](https://www.abcam.com/ps/products/50/ab50212/Images/ab50212-258051-anti-hdac7-antibody-hdac7-97-western-blot.jpg)
Western blot - Anti-HDAC7 antibody [HDAC7-97] (ab50212)Anti-HDAC7 antibody [HDAC7-97] (ab50212) at 0.25 µg/ml + embryonal kidney 293T cells
Predicted band size: 103 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (4)
ab50212 has been referenced in 4 publications.
- Shen W et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . PubMed: 24923522
- Muralidhar SA et al. Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells. J Biol Chem 286:2343-53 (2011). WB ; Human . PubMed: 21078662
- Golding MC et al. Multiple epigenetic modifiers induce aggressive viral extinction in extraembryonic endoderm stem cells. Cell Stem Cell 6:457-67 (2010). ChIP . PubMed: 20452320
- Margariti A et al. Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin. Circ Res 106:1202-11 (2010). PubMed: 20224040
