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    hdac7-antibody-hdac7-97-ab50212.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC7 antibody [HDAC7-97] (ab50212)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-HDAC7 antibody [HDAC7-97] (ab50212)

    Key features and details

    • Mouse monoclonal [HDAC7-97] to HDAC7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Recombinant human HDAC7 protein (ab101660)
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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Product image
    Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade (ab52946)

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    Overview

    • Product name

      Anti-HDAC7 antibody [HDAC7-97]
      See all HDAC7 primary antibodies
    • Description

      Mouse monoclonal [HDAC7-97] to HDAC7
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rat
    • Immunogen

      Synthetic peptide:

      YRTLEPLDTEGAARSVLSS

      conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: human embryonal kidney 293T cells expressing recombinant human HDAC7.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: 0.0268% PBS
    • Concentration information loading...
    • Purification notes

      Ab50212 is a purified IgG.
    • Clonality

      Monoclonal
    • Clone number

      HDAC7-97
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • HDACs
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • HDACs
      • Class II / Hda1 Class
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human HDAC7 protein (ab101660)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab50212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.
    Notes
    WB
    Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.

    Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Domain

      The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
    • Post-translational
      modifications

      May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    • Cellular localization

      Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
    • Target information above from: UniProt accession Q8WUI4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51564 Human
      • Entrez Gene: 84582 Rat
      • Omim: 606542 Human
      • SwissProt: Q8WUI4 Human
      • SwissProt: Q99P96 Rat
      • Unigene: 200063 Human
      • Unigene: 203327 Rat
      • Alternative names

        • DKFZP586J0917 antibody
        • FLJ99588 antibody
        • HD 7a antibody
        • HD7 antibody
        • HD7a antibody
        • HDAC 7 antibody
        • HDAC 7A antibody
        • Hdac7 antibody
        • HDAC7_HUMAN antibody
        • HDAC7A antibody
        • Histone deacetylase 7 antibody
        • Histone deacetylase 7A antibody
        • OTTHUMP00000202813 antibody
        • OTTHUMP00000202814 antibody
        see all

      Images

      • Western blot - Anti-HDAC7 antibody [HDAC7-97] (ab50212)
        Western blot - Anti-HDAC7 antibody [HDAC7-97] (ab50212)
        Anti-HDAC7 antibody [HDAC7-97] (ab50212) at 0.25 µg/ml + embryonal kidney 293T cells

        Predicted band size: 103 kDa

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab50212? Please let us know so that we can cite the reference in this datasheet.

      ab50212 has been referenced in 7 publications.

      • Mei J  et al. OIP5-AS1 modulates epigenetic regulator HDAC7 to enhance non-small cell lung cancer metastasis via miR-140-5p. Oncol Lett 20:7 (2020). PubMed: 32774481
      • Liang H  et al. Orphan nuclear receptor NR4A1 suppresses hyperhomocysteinemia-induced hepatic steatosis in vitro and in vivo. FEBS Lett 593:1061-1071 (2019). PubMed: 30973961
      • Malhotra R  et al. HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nat Genet 51:1580-1587 (2019). PubMed: 31659325
      • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . PubMed: 24923522
      • Muralidhar SA  et al. Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells. J Biol Chem 286:2343-53 (2011). WB ; Human . PubMed: 21078662
      • Golding MC  et al. Multiple epigenetic modifiers induce aggressive viral extinction in extraembryonic endoderm stem cells. Cell Stem Cell 6:457-67 (2010). ChIP . PubMed: 20452320
      • Margariti A  et al. Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin. Circ Res 106:1202-11 (2010). PubMed: 20224040

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-HDAC7 antibody [HDAC7-97] - ChIP Grade

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293T and LNCaP)
      Gel Running Conditions
      Reduced Denaturing (4-15% TGX BioRad)
      Loading amount
      200000 cells
      Specification
      HEK293T and LNCaP
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 01 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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