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Epigenetics and Nuclear Signaling Transcription Other factors
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HDAC8 Activity Assay Kit (Fluorometric) (ab156069)

  • Datasheet
  • SDS
  • Protocol Booklet
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ab156069 - HDAC8 Activity Assay Kit (Fluorometric)
  • ab156069 - HDAC8 Activity Assay Kit (Fluorometric)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Cell culture extracts, Tissue Extracts

Overview

  • Product name

    HDAC8 Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s HDAC8 Activity Assay Kit (Fluorometric) (ab156069) detects HDAC activity in lysates.

    Primarily, the HDAC8 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the HDAC8 Activity Assay Kit (Fluorometric) after immunoprecipitation with an appropriate HDAC8 specific antibody.

    Applications for this kit include:

    1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8 (class I).

    2. Screening inhibitors or activators of HDAC8.

    3. Detecting the effects of pharmacological agents on HDAC8.

  • Notes

    Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

    HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
    Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 100 tests
    Developer 1 x 500µl
    Fluoro-Deacetylated Peptide (0.2 mM) 1 x 100µl
    Fluoro-Substrate Peptide (0.2 mM) 1 x 500µl
    HDAC Assay Buffer 2 x 1ml
    Recombinant HDAC8 1 x 500µl
    Stop Solution 2 x 1ml
    Trichostatin A (200µM) 1 x 500µl
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Kits
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • Histone acetylation and deacetylation
  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Target information above from: UniProt accession Q9BY41 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • HD8
    • HDAC 8
    • Hdac8
    • HDAC8_HUMAN
    • HDACL 1
    • HDACL-1
    • HDACL1
    • Histone deacetylase 8
    • Histone deacetylase like 1
    • RPD 3
    • RPD3
    see all
  • Database links

    • Entrez Gene: 55869 Human
    • SwissProt: Q9BY41 Human
    • Unigene: 310536 Human

    Images

    • ab156069 - HDAC8 Activity Assay Kit (Fluorometric)
      ab156069 - HDAC8 Activity Assay Kit (Fluorometric)
      Dose dependency of recombinant HDAC8 (30min.)
    • ab156069 - HDAC8 Activity Assay Kit (Fluorometric)
      ab156069 - HDAC8 Activity Assay Kit (Fluorometric)
      Time course of HDAC8 reaction

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab156069? Please let us know so that we can cite the reference in this datasheet.

    ab156069 has not yet been referenced specifically in any publications.

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