Recombinant Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
![Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-257912-anti-hdac8-antibody-epr103382-western-blot.jpg "Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10338(2)] to HDAC8
- Suitable for: Flow Cyt (Intra), WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-HDAC8 antibody [EPR10338(2)]
See all HDAC8 primary antibodies -
Description
Rabbit monoclonal [EPR10338(2)] to HDAC8 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Molt4 and K562 cell lysates; human kidney tissue lysate; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10338(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
|
| IP |
1/40 - 1/60.
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| Notes |
|---|
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Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 42 kDa. |
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IP
1/40 - 1/60. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 310536 Human
see all -
Alternative names
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
see all
Images
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Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HADC8 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220298-anti-hdac8-antibody-epr103382-western-blot.jpg)
Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)All lanes : Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa -
![Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220297-anti-hdac8-antibody-epr103382-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.
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![Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220296-anti-hdac8-antibody-epr103382-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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![Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-5-benefits-of-recombinant-antibodies.png)
Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (19)
ab187139 has been referenced in 19 publications.
- Wang J et al. HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice. Int J Mol Med 45:1150-1162 (2020). PubMed: 32124940
- Zhang R et al. HDAC8-dependent deacetylation of PKM2 directs nuclear localization and glycolysis to promote proliferation in hepatocellular carcinoma. Cell Death Dis 11:1036 (2020). PubMed: 33279948
- Aboukhatwa SM et al. Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue. ChemMedChem 14:1096-1107 (2019). PubMed: 30921497
- Buckwalter JM et al. Characterization of Histone Deacetylase Expression Within In Vitro and In Vivo Bladder Cancer Model Systems. Int J Mol Sci 20:N/A (2019). PubMed: 31137849
- Teixeira PDS et al. Brain STAT5 Modulates Long-Term Metabolic and Epigenetic Changes Induced by Pregnancy and Lactation in Female Mice. Endocrinology 160:2903-2917 (2019). PubMed: 31599926
