Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-HDAC8 antibody [EPR10338(2)]
See all HDAC8 primary antibodies -
DescriptionRabbit monoclonal [EPR10338(2)] to HDAC8
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human HDAC8 aa 50-150. The exact sequence is proprietary.
Database link: Q9BY41 -
Positive control
- HeLa, Molt4 and K562 cell lysates; human kidney tissue lysate; K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10338(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab187139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 42 kDa. | |
| Flow Cyt | 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/40 - 1/60. |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
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Tissue specificityWeakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
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Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
- Information by UniProt
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Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 310536 Human
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Alternative names
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
see all
Images
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![Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-257912-ab187139KOWB.jpg)
Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HADC8 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220298-ab187139-wb.jpg)
Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)All lanes : Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa -
![Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220297-ab187139-ip.jpg)
Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.
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![Flow Cytometry - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)](https://www.abcam.com/ps/products/187/ab187139/Images/ab187139-220296-ab187139-fc.jpg)
Flow Cytometry - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)Flow cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
References
This product has been referenced in:
- Wang P et al. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1ß-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways. Int J Mol Med 41:2117-2127 (2018). Read more (PubMed: 29393346) »
- Yang M et al. I-7ab inhibited the growth of TNBC cells via targeting HDAC3 and promoting the acetylation of p53. Biomed Pharmacother 99:220-226 (2018). WB . Read more (PubMed: 29334665) »
Customer reviews and Q&As
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