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  1. Link

    hdac8-antibody-epr103382-ab187139.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (21)

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Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
  • Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10338(2)] to HDAC8
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

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Carrier Free

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Overview

  • Product name

    Anti-HDAC8 antibody [EPR10338(2)]
    See all HDAC8 primary antibodies
  • Description

    Rabbit monoclonal [EPR10338(2)] to HDAC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Molt4 and K562 cell lysates; human kidney tissue lysate; K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10338(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
  • Assay kits

    • HDAC8 Activity Assay Kit (Fluorometric) (ab156069)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human HDAC8 protein (ab42633)
  • Related Products

    • Recombinant Human HDAC8 protein (ab42633)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IP
1/40 - 1/60.
Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IP
1/40 - 1/60.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Target information above from: UniProt accession Q9BY41 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55869 Human
    • Entrez Gene: 70315 Mouse
    • Entrez Gene: 363481 Rat
    • Omim: 300269 Human
    • SwissProt: Q9BY41 Human
    • SwissProt: Q8VH37 Mouse
    • SwissProt: B1WC68 Rat
    • Unigene: 310536 Human
    • Unigene: 328128 Mouse
    • Unigene: 208476 Rat
    see all
  • Alternative names

    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HADC8 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
    ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    All lanes : Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Molt4 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.

  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG. 

     

     

  • Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
    Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

Protocols

  • Western blot protocols
  • Flow cytometry protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (21)

Publishing research using ab187139? Please let us know so that we can cite the reference in this datasheet.

ab187139 has been referenced in 21 publications.

  • Chen Y  et al. HDAC8 drives spindle organization during meiotic maturation of porcine oocytes. Cell Prolif 54:e13119 (2021). PubMed: 34435400
  • Wang J  et al. HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice. Int J Mol Med 45:1150-1162 (2020). PubMed: 32124940
  • Zhang R  et al. HDAC8-dependent deacetylation of PKM2 directs nuclear localization and glycolysis to promote proliferation in hepatocellular carcinoma. Cell Death Dis 11:1036 (2020). PubMed: 33279948
  • Singh VP  et al. Oocyte-specific deletion of Hdac8 in mice reveals stage-specific effects on fertility. Reproduction 157:305-316 (2019). PubMed: 30620721
  • Aboukhatwa SM  et al. Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue. ChemMedChem 14:1096-1107 (2019). PubMed: 30921497
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-HDAC8 antibody [EPR10338(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
12 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Apr 02 2016

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