Overview

  • Product name
    Anti-HDAC8 antibody - N-terminal
    See all HDAC8 primary antibodies
  • Description
    Rabbit polyclonal to HDAC8 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human HDAC8 aa 18-33 (N terminal).
    Sequence:

    YIYSPEYVSMCDSLAK


    Database link: Q9BY41

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Rat Cardiac Muscle tissue Lysate, A549 Cell Lysate; Human Lung Cancer Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab190954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity
    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization
    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)

    Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labeling HDAC8 with ab190954 at 1 µg/ml.

  • Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)
    Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)
    All lanes : Anti-HDAC8 antibody - N-terminal (ab190954) at 0.5 µg/ml

    Lane 1 : Rat Cardiac Muscle tissue Lysate
    Lane 2 : A549 Cell Lysate

    Predicted band size: 42 kDa

References

ab190954 has not yet been referenced specifically in any publications.

Publishing research using ab190954? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab190954.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up