Anti-HDAC8 antibody - N-terminal (ab190954)
Key features and details
- Rabbit polyclonal to HDAC8 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC8 antibody - N-terminal
See all HDAC8 primary antibodies -
Description
Rabbit polyclonal to HDAC8 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
-
Immunogen
Synthetic peptide corresponding to Human HDAC8 aa 1-100 (N terminal).
Database link: Q9BY41 -
Positive control
- WB: Rat cardiac muscle tissue, A549 whole cell lysate; IHC-P: Human lung cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Rat
|
| All applications |
Mouse
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
|
|
| IHC-P |
Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa. |
|
IHC-P
Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 310536 Human
see all -
Alternative names
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
see all
Images
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Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)All lanes : Anti-HDAC8 antibody - N-terminal (ab190954) at 0.5 µg/ml
Lane 1 : Rat cardiac muscle tissue lysate at 50 µg
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 40 µg
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling HDAC8 with ab190954 at 1 µg/ml.
Protocols
References (0)
ab190954 has not yet been referenced specifically in any publications.
