Overview

  • Product name
    Anti-HDAC8 antibody - N-terminal
    See all HDAC8 primary antibodies
  • Description
    Rabbit polyclonal to HDAC8 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human HDAC8 aa 18-33 (N terminal).
    Sequence:

    YIYSPEYVSMCDSLAK


    Database link: Q9BY41

  • Positive control
    • Rat Cardiac Muscle tissue Lysate, A549 Cell Lysate; Human Lung Cancer Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab190954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity
    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization
    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labeling HDAC8 with ab190954 at 1 µg/ml.

  • All lanes : Anti-HDAC8 antibody - N-terminal (ab190954) at 0.5 µg/ml

    Lane 1 : Rat Cardiac Muscle tissue Lysate
    Lane 2 : A549 Cell Lysate

    Predicted band size: 42 kDa

References

ab190954 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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