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    hdac8-antibody-n-terminal-ab190954.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC8 antibody - N-terminal (ab190954)

  • Datasheet
  • SDS
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Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)

Key features and details

  • Rabbit polyclonal to HDAC8 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant Human HDAC8 protein (ab42633)

View more associated products

Overview

  • Product name

    Anti-HDAC8 antibody - N-terminal
    See all HDAC8 primary antibodies
  • Description

    Rabbit polyclonal to HDAC8 - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human HDAC8 aa 1-100 (N terminal).
    Database link: Q9BY41

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat cardiac muscle tissue, A549 whole cell lysate; IHC-P: Human lung cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human HDAC8 protein (ab42633)
  • Related Products

    • Recombinant Human HDAC8 protein (ab42633)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Rat
All applications
Mouse
Cow
Application Abreviews Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P
Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P
Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Target information above from: UniProt accession Q9BY41 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55869 Human
    • Entrez Gene: 70315 Mouse
    • Entrez Gene: 363481 Rat
    • Omim: 300269 Human
    • SwissProt: Q9BY41 Human
    • SwissProt: Q8VH37 Mouse
    • SwissProt: B1WC68 Rat
    • Unigene: 310536 Human
    • Unigene: 328128 Mouse
    • Unigene: 208476 Rat
    see all
  • Alternative names

    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)
    Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)
    All lanes : Anti-HDAC8 antibody - N-terminal (ab190954) at 0.5 µg/ml

    Lane 1 : Rat cardiac muscle tissue lysate at 50 µg
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 40 µg

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling HDAC8 with ab190954 at 1 µg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab190954? Please let us know so that we can cite the reference in this datasheet.

    ab190954 has not yet been referenced specifically in any publications.

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