Anti-HDAC8 antibody - N-terminal (ab190954)
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Overview
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Product nameAnti-HDAC8 antibody - N-terminal
See all HDAC8 primary antibodies -
DescriptionRabbit polyclonal to HDAC8 - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide corresponding to Human HDAC8 aa 18-33 (N terminal).
Sequence:YIYSPEYVSMCDSLAK
Database link: Q9BY41 -
Positive control
- Rat Cardiac Muscle tissue Lysate, A549 Cell Lysate; Human Lung Cancer Tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab190954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa. | |
| IHC-P | Use 0.5-1µg for 10 cells. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
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Tissue specificityWeakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
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Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
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Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
- Information by UniProt
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Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 310536 Human
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Alternative names
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
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Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody - N-terminal (ab190954)Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labeling HDAC8 with ab190954 at 1 µg/ml.
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Western blot - Anti-HDAC8 antibody - N-terminal (ab190954)All lanes : Anti-HDAC8 antibody - N-terminal (ab190954) at 0.5 µg/ml
Lane 1 : Rat Cardiac Muscle tissue Lysate
Lane 2 : A549 Cell Lysate
Predicted band size: 42 kDa
References
ab190954 has not yet been referenced specifically in any publications.
