Anti-HDAC8 (phospho S39) antibody (ab131516)
Key features and details
- Rabbit polyclonal to HDAC8 (phospho S39)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC8 (phospho S39) antibody
See all HDAC8 primary antibodies -
Description
Rabbit polyclonal to HDAC8 (phospho S39) -
Host species
Rabbit -
Specificity
ab131516 detects endogenous levels of HDAC8 only when phosphorylated at serine 39. -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
Immunogen
Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of serine 39 (RASpMV) derived from Human HDAC8 (NP_001159890.1).
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Positive control
- HeLa cells; Human lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131516 was purified by affinity chromatography using epitope specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 310536 Human
see all -
Alternative names
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
see all
Images
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling HDAC8 with ab131516 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling HDAC8 with ab131516 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131516 has not yet been referenced specifically in any publications.