Anti-HDAC8 (phospho S39) antibody (ab131516)

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Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 (phospho S39) antibody (ab131516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)

Key features and details

  • Rabbit polyclonal to HDAC8 (phospho S39)
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HDAC8 (phospho S39) antibody
    See all HDAC8 primary antibodies

  • Description

    Rabbit polyclonal to HDAC8 (phospho S39)

  • Host species

    Rabbit

  • Specificity

    ab131516 detects endogenous levels of HDAC8 only when phosphorylated at serine 39.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of serine 39 (RASpMV) derived from Human HDAC8 (NP_001159890.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa cells; Human lung carcinoma tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.
Notes
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.

  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling HDAC8 with ab131516 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling HDAC8 with ab131516 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References (0)

Publishing research using ab131516? Please let us know so that we can cite the reference in this datasheet.

ab131516 has not yet been referenced specifically in any publications.

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