Overview

  • Product name
    Anti-HDAC8 (phospho S39) antibody
    See all HDAC8 primary antibodies
  • Description
    Rabbit polyclonal to HDAC8 (phospho S39)
  • Host species
    Rabbit
  • Specificity
    ab131516 detects endogenous levels of HDAC8 only when phosphorylated at serine 39.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of serine 39 (RASpMV) derived from Human HDAC8 (NP_001159890.1).

  • Positive control
    • HeLa cells; Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity
    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization
    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling HDAC8 with ab131516 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling HDAC8 with ab131516 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

ab131516 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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