Anti-HDAC8 (phospho S39) antibody (ab131516)

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Overview

  • Product name
    Anti-HDAC8 (phospho S39) antibody
    See all HDAC8 primary antibodies
  • Description
    Rabbit polyclonal to HDAC8 (phospho S39)
  • Host species
    Rabbit
  • Specificity
    ab131516 detects endogenous levels of HDAC8 only when phosphorylated at serine 39.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of serine 39 (RASpMV) derived from Human HDAC8 (NP_001159890.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa cells; Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity
    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization
    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling HDAC8 with ab131516 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 (phospho S39) antibody (ab131516)
    Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling HDAC8 with ab131516 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

ab131516 has not yet been referenced specifically in any publications.

Publishing research using ab131516? Please let us know so that we can cite the reference in this datasheet.

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