Product nameAnti-HDAC8 (phospho S39) antibody
See all HDAC8 primary antibodies
DescriptionRabbit polyclonal to HDAC8 (phospho S39)
SpecificityThis antibody is specific for HDAC8 only when phosphorylated at serine 39.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide derived from Human HDAC8 around the phosphorylation site of serine 39 (RASpMV).
- Human lung carcinoma tissue slides.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab38514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
Tissue specificityWeakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
- Information by UniProt
- CDA07 antibody
- CDLS5 antibody
- HD 8 antibody
ab38514 has not yet been referenced specifically in any publications.