Recombinant Anti-HDAC9 antibody [EPR5223] (ab109446)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5223] to HDAC9
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-HDAC9 antibody [EPR5223]
See all HDAC9 primary antibodies -
Description
Rabbit monoclonal [EPR5223] to HDAC9 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human HDAC9 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- IHC-P: Human colon Tissue ICC/IF: HeLa cells WB: HepG2, Raji, HAP1, cell lysates Flow Cyt: K-562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5223 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. -
Tissue specificity
Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). -
Involvement in disease
Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Post-translational
modificationsPhosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Sumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9734 Human
- Entrez Gene: 687001 Rat
- Omim: 606543 Human
- SwissProt: Q9UKV0 Human
- Unigene: 196054 Human
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Alternative names
- HD 7 antibody
- HD 7B antibody
- HD 9 antibody
see all
Images
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![Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)](https://www.abcam.com/ps/products/109/ab109446/Images/ab109446-279680-anti-hdac9-antibody-epr5223-western-blot.jpg)
Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109446 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109446 was shown to specifically recognize HDAC9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HDAC9 knockout samples were usedexamined. Wild-type and HDAC9 knockout samples were subjected to SDS-PAGE. Ab109446 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)](https://www.abcam.com/ps/products/109/ab109446/Images/ab109446-122308-anti-hdac9-antibody-epr5223-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (ab109446)](https://www.abcam.com/ps/products/109/ab109446/Images/ab109446-122307-anti-hdac9-antibody-epr5223-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (ab109446)ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence. -
![Flow Cytometry - Anti-HDAC9 antibody [EPR5223] (ab109446)](https://www.abcam.com/ps/products/109/ab109446/Images/ab109446-308655-anti-hdac9-antibody-epr5223-flow-cytometry.jpg)
Flow Cytometry - Anti-HDAC9 antibody [EPR5223] (ab109446)Flow cytometry of K-562 Human chronic myelogenous leukemia lymphoblast cells staining HDAC9 with ab109446 (red) at 1:250. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used at 1:2000 as a secondary. Rabbit monoclonal IgG (ab172730) (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) used as an unlabelled control. Fixed with 4% paraformaldehyde. Permeabilised with 90% methanol.
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![Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)](https://www.abcam.com/ps/products/109/ab109446/Images/ab109446-122309-anti-hdac9-antibody-epr5223-western-blot.jpg)
Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 111 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References (9)
ab109446 has been referenced in 9 publications.
- Li H et al. High HDAC9 is associated with poor prognosis and promotes malignant progression in pancreatic ductal adenocarcinoma. Mol Med Rep 21:822-832 (2020). PubMed: 31974610
- Fu Y et al. Decreased expression and hypomethylation of HDAC9 lead to poor prognosis and inhibit immune cell infiltration in clear cell renal cell carcinoma. Urol Oncol N/A:N/A (2020). PubMed: 32402768
- Li A et al. Histone deacetylase 6 regulates the immunosuppressive properties of cancer-associated fibroblasts in breast cancer through the STAT3-COX2-dependent pathway. Oncogene N/A:N/A (2018). PubMed: 29980788
- Jin Q et al. MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16:1663-1670 (2018). PubMed: 30186385
- Di Giorgio E et al. The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness. PLoS Genet 13:e1006752 (2017). WB, ChIP ; Human . PubMed: 28419090
- Bourgeois CT et al. HDAC9 is an epigenetic repressor of kidney angiotensinogen establishing a sex difference. Biol Sex Differ 8:18 (2017). PubMed: 28572913
- Oksala NKJ et al. Synergistic Expression of Histone Deacetylase 9 and Matrix Metalloproteinase 12 in M4 Macrophages in Advanced Carotid Plaques. Eur J Vasc Endovasc Surg 53:632-640 (2017). IHC-P ; Human . PubMed: 28343758
- Sebastian S et al. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes Dev 27:1247-59 (2013). PubMed: 23723416
- Klinz FJ et al. Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo. Histochem Cell Biol : (2012). ICC/IF, IHC-FrFl ; Human . PubMed: 22297573
