• Product name

    Anti-HDAC9 antibody [EPR5223]
    See all HDAC9 primary antibodies
  • Description

    Rabbit monoclonal [EPR5223] to HDAC9
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human HDAC9 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human colon tissue ICC/IF: HeLa cells WB: HepG2, Raji, HAP1, cell lysates FC: K-562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • Tissue specificity

      Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • Involvement in disease

      Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Post-translational

      Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • HD 7 antibody
      • HD 7B antibody
      • HD 9 antibody
      • HD7 antibody
      • HD7B antibody
      • HD9 antibody
      • HDAC 7 antibody
      • HDAC 7B antibody
      • HDAC 9 antibody
      • HDAC 9B antibody
      • HDAC 9FL antibody
      • HDAC antibody
      • HDAC7 antibody
      • HDAC7B antibody
      • HDAC9 antibody
      • HDAC9_HUMAN antibody
      • HDAC9B antibody
      • HDAC9FL antibody
      • HDRP antibody
      • Histone deacetylase 4/5 related protein antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7B antibody
      • Histone deacetylase 9 antibody
      • Histone deacetylase 9A antibody
      • Histone deacetylase related protein antibody
      • Histone deacetylase-related protein antibody
      • KIAA0744 antibody
      • MEF2 interacting transcription repressor MITR antibody
      • MEF2 interacting transcription repressor protein antibody
      • MEF2-interacting transcription repressor MITR antibody
      • MITR antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109446 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109446 was shown to specifically recognize HDAC9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HDAC9 knockout samples were usedexamined. Wild-type and HDAC9 knockout samples were subjected to SDS-PAGE. Ab109446 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 111 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?

    • ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.
    • Flow cytometry of K-562 Human chronic myelogenous leukemia lymphoblast cells staining HDAC9 with ab109446 (red) at 1:250. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used at 1:2000 as a secondary. Rabbit monoclonal IgG (ab172730)  (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) used as an unlabelled control. Fixed with 4% paraformaldehyde. Permeabilised with 90% methanol.


    This product has been referenced in:

    • Li A  et al. Histone deacetylase 6 regulates the immunosuppressive properties of cancer-associated fibroblasts in breast cancer through the STAT3-COX2-dependent pathway. Oncogene N/A:N/A (2018). Read more (PubMed: 29980788) »
    • Jin Q  et al. MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16:1663-1670 (2018). Read more (PubMed: 30186385) »
    See all 7 Publications for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections)
    Rat Tissue sections (Basilar Artery)
    Yes - 0.1% Triton-X PBS
    Basilar Artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Courtney Fisher

    Verified customer

    Submitted Apr 19 2017

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