Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

Overview

  • Product name

    Anti-HDAC9 antibody [EPR5223] - BSA and Azide free
    See all HDAC9 primary antibodies
  • Description

    Rabbit monoclonal [EPR5223] to HDAC9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human HDAC9 aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab239979 is the carrier-free version of ab109446. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239979 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • Tissue specificity

      Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • Involvement in disease

      Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Post-translational
      modifications

      Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Sumoylated.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HD 7 antibody
      • HD 7B antibody
      • HD 9 antibody
      • HD7 antibody
      • HD7B antibody
      • HD9 antibody
      • HDAC 7 antibody
      • HDAC 7B antibody
      • HDAC 9 antibody
      • HDAC 9B antibody
      • HDAC 9FL antibody
      • HDAC antibody
      • HDAC7 antibody
      • HDAC7B antibody
      • HDAC9 antibody
      • HDAC9_HUMAN antibody
      • HDAC9B antibody
      • HDAC9FL antibody
      • HDRP antibody
      • Histone deacetylase 4/5 related protein antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7B antibody
      • Histone deacetylase 9 antibody
      • Histone deacetylase 9A antibody
      • Histone deacetylase related protein antibody
      • Histone deacetylase-related protein antibody
      • KIAA0744 antibody
      • MEF2 interacting transcription repressor MITR antibody
      • MEF2 interacting transcription repressor protein antibody
      • MEF2-interacting transcription repressor MITR antibody
      • MITR antibody
      see all

    References

    ab239979 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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