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    hdac9-antibody-n-terminal-ab211471.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-HDAC9 antibody - N-terminal (ab211471)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody - N-terminal (ab211471)

    Key features and details

    • Rabbit polyclonal to HDAC9 - N-terminal
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human HDAC9 protein (ab80350)

    View more associated products

    Overview

    • Product name

      Anti-HDAC9 antibody - N-terminal
      See all HDAC9 primary antibodies
    • Description

      Rabbit polyclonal to HDAC9 - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human HDAC9 (N terminal). NP_055522.1.
      Database link: Q9UKV0-3

    • Positive control

      • Human small intestine tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Constituents: 30% Glycerol, 0.42% Potassium phosphate, 0.87% Sodium chloride

      Sterile filtered
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • HDACs
      • Class II / Hda1 Class
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human HDAC9 protein (ab80350)

    Applications

    Our Abpromise guarantee covers the use of ab211471 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • Tissue specificity

      Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • Involvement in disease

      Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 2 subfamily.
    • Post-translational
      modifications

      Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Sumoylated.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UKV0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9734 Human
      • Omim: 606543 Human
      • SwissProt: Q9UKV0 Human
      • Unigene: 196054 Human
      • Alternative names

        • HD 7 antibody
        • HD 7B antibody
        • HD 9 antibody
        • HD7 antibody
        • HD7B antibody
        • HD9 antibody
        • HDAC 7 antibody
        • HDAC 7B antibody
        • HDAC 9 antibody
        • HDAC 9B antibody
        • HDAC 9FL antibody
        • HDAC antibody
        • HDAC7 antibody
        • HDAC7B antibody
        • HDAC9 antibody
        • HDAC9_HUMAN antibody
        • HDAC9B antibody
        • HDAC9FL antibody
        • HDRP antibody
        • Histone deacetylase 4/5 related protein antibody
        • Histone deacetylase 7 antibody
        • Histone deacetylase 7B antibody
        • Histone deacetylase 9 antibody
        • Histone deacetylase 9A antibody
        • Histone deacetylase related protein antibody
        • Histone deacetylase-related protein antibody
        • KIAA0744 antibody
        • MEF2 interacting transcription repressor MITR antibody
        • MEF2 interacting transcription repressor protein antibody
        • MEF2-interacting transcription repressor MITR antibody
        • MITR antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody - N-terminal (ab211471)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody - N-terminal (ab211471)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling HDAC9 with ab211471 at 5 µg/ml.

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab211471? Please let us know so that we can cite the reference in this datasheet.

      ab211471 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-HDAC9 antibody - N-terminal

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - nuclear (RAW264.7 Macrophage)
      Gel Running Conditions
      Reduced Denaturing (4-16%)
      Loading amount
      20 µg
      Treatment
      LPS for 1-4hr
      Specification
      RAW264.7 Macrophage
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 23 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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