Key features and details
- Mouse monoclonal [26C4a] to HDGF
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HDGF antibody [26C4a]
See all HDGF primary antibodies
DescriptionMouse monoclonal [26C4a] to HDGF
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment of human HDGF, from an internal sequence.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab58644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).|
|Dot blot||Use at an assay dependent concentration.|
FunctionHeparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
Sequence similaritiesBelongs to the HDGF family.
Contains 1 PWWP domain.
DomainThe PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
modificationsSumoylated by SUMO1. Sumoylation prevents binding to chromatin.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein
at 25 µg
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The immunogen shown in the WB is tagged.
ab58644 has been referenced in 2 publications.
- Leich E et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P ; Human . PubMed: 21960592
- Maschietto M et al. Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Cell Death Dis 2:e224 (2011). PubMed: 22048167