Product nameAnti-HDGF antibody [EPR7898]
See all HDGF primary antibodies
DescriptionRabbit monoclonal [EPR7898] to HDGF
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HDGF aa 100-200. The exact sequence is proprietary.
- HeLa, A549, SKBR-3 and ECV-304 cell lysates; Human hepatocellular carcinoma and Human colon tissues
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionHeparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
Sequence similaritiesBelongs to the HDGF family.
Contains 1 PWWP domain.
DomainThe PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
modificationsSumoylated by SUMO1. Sumoylation prevents binding to chromatin.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
All lanes : Anti-HDGF antibody [EPR7898] (ab128921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
ab128921 staining HDGF in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
Overlay histogram showing HeLa cells stained with ab128921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human hepatocellular carcinoma tissue by Immunohistochemistry.
ab128921, at a 1/100 dilution, staining HDGF in paraffin embedded Human colon tissue by Immunohistochemistry.
This product has been referenced in:
- Zheng Y et al. Long non-coding RNA AGAP2-AS1 promotes the proliferation of glioma cells by sponging miR-15a/b-5p to upregulate the expression of HDGF and activating Wnt/ß-catenin signaling pathway. Int J Biol Macromol 128:521-530 (2019). Read more (PubMed: 30684575) »