Recombinant
RabMAb

Recombinant Anti-HDGF antibody [EPR7898] (HRP) (ab201820)

Overview

  • Product name

    Anti-HDGF antibody [EPR7898] (HRP)
    See all HDGF primary antibodies
  • Description

    Rabbit monoclonal [EPR7898] to HDGF (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human HDGF aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: A549 and SKBR-3 whole cell lysates. IHC-P: FFPE human placenta (normal) tissue sections.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7898
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
  • Post-translational
    modifications

    Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ96580 antibody
    • HDGF antibody
    • HDGF_HUMAN antibody
    • Hepatoma derived growth factor antibody
    • Hepatoma-derived growth factor antibody
    • High mobility group protein 1 like 2 antibody
    • High mobility group protein 1-like 2 antibody
    • HMG-1L2 antibody
    • HMG1L2 antibody
    see all

Images

  • IHC image of HDGF staining in a section of formalin-fixed paraffin-embedded human placenta (normal)*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201820, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-HDGF antibody [EPR7898] (HRP) (ab201820) at 1/1000 dilution + SKBR3 (Human breat adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201820 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Anti-HDGF antibody [EPR7898] (HRP) (ab201820) at 1/1000 dilution + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201820 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

     

References

ab201820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201820.
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