Recombinant
RabMAb

Recombinant Anti-HDGF antibody [EPR7899] (ab131046)

Overview

  • Product name

    Anti-HDGF antibody [EPR7899]
    See all HDGF primary antibodies
  • Description

    Rabbit monoclonal [EPR7899] to HDGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C terminus of Human HDGF (UniProt P51858).

  • Positive control

    • A549, Saos-2, ECV-304, and 293T cell lysates; A549 cells; Human colon tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7899
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the HDGF family.
      Contains 1 PWWP domain.
    • Domain

      The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
    • Post-translational
      modifications

      Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ96580 antibody
      • HDGF antibody
      • HDGF_HUMAN antibody
      • Hepatoma derived growth factor antibody
      • Hepatoma-derived growth factor antibody
      • High mobility group protein 1 like 2 antibody
      • High mobility group protein 1-like 2 antibody
      • HMG-1L2 antibody
      • HMG1L2 antibody
      see all

    Images

    • All lanes : Anti-HDGF antibody [EPR7899] (ab131046) at 1/10000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : ECV-304 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HDGF with ab131046 antibody at 1/250 dilution.
    • Immunofluorescence analysis of A549 cells labelling HDGF with ab131046 antibody at 1/250 dilution.

    References

    ab131046 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer cell lines total lysate)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Specification
    Cancer cell lines total lysate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Dec 07 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Specification
    U2OS cells
    Treatment
    siLuc and siHDGF
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Sep 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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