Recombinant Anti-HDGF antibody [EPR7899] (ab131046)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7899] to HDGF
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HDGF antibody [EPR7899]
See all HDGF primary antibodies -
Description
Rabbit monoclonal [EPR7899] to HDGF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549, Saos-2, ECV-304, and 293T cell lysates; A549 cells; Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7899 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the HDGF family.
Contains 1 PWWP domain. -
Domain
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured. -
Post-translational
modificationsSumoylated by SUMO1. Sumoylation prevents binding to chromatin. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3068 Human
- Omim: 600339 Human
- SwissProt: P51858 Human
- Unigene: 506748 Human
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Alternative names
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
see all
Images
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All lanes : Anti-HDGF antibody [EPR7899] (ab131046) at 1/10000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HDGF with ab131046 antibody at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of A549 cells labelling HDGF with ab131046 antibody at 1/250 dilution.
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Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling HDGF with purified ab131046 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131046 has not yet been referenced specifically in any publications.