Overview

  • Product name

  • Description

    Mouse polyclonal to HDHD2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    HDHD2 (NP_115500, 1 a.a. ~ 259 a.a) human protein.

  • Positive control

    • transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Sequence similarities

    Belongs to the HAD-like hydrolase superfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610039H12Rik antibody
    • 3110052N05Rik antibody
    • DKFZP564D1378 antibody
    • Haloacid dehalogenase like hydrolase domain containing 2 antibody
    • Haloacid dehalogenase-like hydrolase domain-containing protein 2 antibody
    • HDHD2 antibody
    • HDHD2_HUMAN antibody
    • Ier3ip1 antibody
    • Immediate early response 3 interacting protein 1 antibody
    • LRRG00122 antibody
    • OTTMUSP00000045339 antibody
    • OTTMUSP00000045340 antibody
    • OTTMUSP00000045341 antibody
    • OTTMUSP00000045343 antibody
    • OTTMUSP00000045344 antibody
    • RGD1308579 antibody
    see all

Images

  • All lanes : Anti-HDHD2 antibody (ab69274) at 1/500 dilution

    Lane 1 : HDHD2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

References

ab69274 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Tris-glycine SDS PAGE gel)
Sample
Mouse Tissue lysate - whole (protein extract of WT C57BL6/J mouse thymus)
Specification
protein extract of WT C57BL6/J mouse thymus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Jung Eun Choi

Verified customer

Submitted Jul 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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