Key features and details
- Mouse monoclonal [OTI1C5] to HDHD3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-HDHD3 antibody [OTI1C5]
See all HDHD3 primary antibodies
DescriptionMouse monoclonal [OTI1C5] to HDHD3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human HDHD3 aa 1-251. Produced in E.coli (NP_112496).
MAHRLQIRLLTWDVKDTLLRLRHPLGEAYATKARAHGLEVEPSALEQGFR QAYRAQSHSFPNYGLSHGLTSRQWWLDVVLQTFHLAGVQDAQAVAPIAEQ LYKDFSHPCTWQVLDGAEDTLRECRTRGLRLAVISNFDRRLEGILGGLGL REHFDFVLTSEAAGWPKPDPRIFQEALRLAHMEPVVAAHVGDNYLCDYQG PRAVGMHSFLVVGPQALDPVVRDSVPKEHILPSLAHLLPALDCLEGSTPG L
Database link: Q9BSH5
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY HDHD3 cDNA for 48 hrs. IHC-P: Human breast, liver, liver carcinoma, pancreas and appendix tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 28 kDa.|
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.5 before commencing with IHC staining protocol.
Sequence similaritiesBelongs to the HAD-like hydrolase superfamily.
- Information by UniProt
- 2810435D12Rik antibody
- C9orf158 antibody
- Chromosome 9 open reading frame 158 antibody
All lanes : Anti-HDHD3 antibody [OTI1C5] (ab236389) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY HDHD3 cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 28 kDa
Paraffin-embedded human breast tissue stained for HDHD3 using ab236389 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for HDHD3 using ab236389 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human liver carcinoma tissue stained for HDHD3 using ab236389 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for HDHD3 using ab236389 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human appendix tissue stained for HDHD3 using ab236389 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236389 has not yet been referenced specifically in any publications.