Overview

  • Product name

    HDL Uptake Assay Kit (Fluorometric)
    See all HDL kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    HDL Uptake Assay Kit (Fluorometric) (ab204717) contains a fluorescently-labeled HDL that can be measured when taken up by cells. Unlabeled-HDL is included in the kit for assay validation.

  • Notes

    High-density lipoprotein (HDL) consists of a protein shell, containing apolipoproteins A-I and A-II, and a hydrophobic core consisting of cholesterol and triglycerides surrounded by phospholipids and cholesterol esters. HDL transports cholesterol to the liver or steroidogenic organs such as adrenals, ovary, and testes by both direct and indirect pathways. HDL-Cholesterol is removed by HDL receptors such as scavenger receptor BI (SR-BI), which mediate the selective uptake of cholesterol from HDL. Clinically, high levels of HDL has been associated with cardiovascular health. This is due to the ability of HDL particle to transport cholesterol from lipid-laden macrophages of atherosclerotic arteries to the liver for secretion into the bile by a process called as reverse cholesterol transport.

  • Platform

    Microplate reader

Properties

Images

  • Fluorescently-labeled HDL Standard curve.

  • Fluorescently-labeled HDL uptake in HepG2 (hepatocytes) cells. Cells were starved for 4 hrs and incubated with Fluorescently-labeled HDL (10 µg) for 4 hrs. Fluorescently-labeled HDL (HDL) uptake was inhibited by SR-BI (HDL receptor) antibody (HDL + SRB1 Ab1), BLT1 (SR-BI Inhibitor; HDL + BLT1) and outcompeted by Unlabeled-HDL (HDL+ UL-HDL [20 µg]). Background was subtracted from the data (Wash off was subtracted as background).

Protocols

References

This product has been referenced in:

  • Huang Y  et al. Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5. Front Neurol 10:32 (2019). Read more (PubMed: 30778327) »
  • Zhu L  et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). Read more (PubMed: 29331506) »
See all 2 Publications for this product

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