Key features and details
- Rabbit polyclonal to HDPTP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HDPTP antibody
DescriptionRabbit polyclonal to HDPTP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gave a positive signal in HDPTP transfected 293T whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab12553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Contains 1 BRO1 domain.
Contains 2 TPR repeats.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic vesicle. Endosome. Cytoplasm > cytoskeleton > cilium basal body.
- Information by UniProt
- DKFZP564F0923 antibody
- EC 22.214.171.124 antibody
- HD PTP antibody
ICC/IF image of ab12553 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12553 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-HDPTP antibody (ab12553) at 1 µg/ml + PTPN23 293T cell transient overexpression lysate (denatured) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 179 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab12553 has not yet been referenced specifically in any publications.