Product nameAnti-HDPTP antibody
DescriptionRabbit polyclonal to HDPTP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gave a positive signal in HDPTP transfected 293T whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab12553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Contains 1 BRO1 domain.
Contains 2 TPR repeats.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic vesicle. Endosome. Cytoplasm > cytoskeleton > cilium basal body.
- Information by UniProt
- DKFZP564F0923 antibody
- EC 18.104.22.168 antibody
- HD PTP antibody
ICC/IF image of ab12553 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12553 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-HDPTP antibody (ab12553) at 1 µg/ml + PTPN23 293T cell transient overexpression lysate (denatured) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 179 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab12553 has not yet been referenced specifically in any publications.