- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-HE4 antibody
See all HE4 primary antibodies -
DescriptionRabbit polyclonal to HE4
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human HE4.
(Peptide available as ab98946.)
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human lung; Human ovary.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab85179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Tissue specificityExpressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
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Sequence similaritiesContains 2 WAP domains.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 10406 Human
- SwissProt: Q14508 Human
- Unigene: 2719 Human
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FormThere are 5 isoforms produced by alternative splicing.
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Alternative names
- dJ461P17.6 antibody
- EDDM4 antibody
- epididymal protein 4 antibody
see all
Images
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Western blot - Anti-HE4 antibody (ab85179)All lanes : Anti-HE4 antibody (ab85179) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 14 kDa (why is the actual band size different from the predicted?)
Exposure time: 16 minutes
Human WAP four-disulfide core domain protein 2 precursor (HE4) contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-HE4 antibody (ab85179)ICC/IF image of ab85179 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
References
This product has been referenced in:
- Markovic-Lipkovski J et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). ICC/IF ; Human . Read more (PubMed: 26327314) »
- Eade CR et al. HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria. Protein Pept Lett 22:672-80 (2015). Read more (PubMed: 25751267) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"