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    he4-antibody-ab85179.pdf

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Anti-HE4 antibody (ab85179)

  • Datasheet
Reviews (1) Submit a question References (2)

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Immunocytochemistry - Anti-HE4 antibody (ab85179)
  • Western blot - Anti-HE4 antibody (ab85179)

Key features and details

  • Rabbit polyclonal to HE4
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Primary
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Protein
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Recombinant Human HE4 protein (ab184603)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-HE4 antibody
    See all HE4 primary antibodies
  • Description

    Rabbit polyclonal to HE4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human lung and MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human HE4 protein (ab184603)

Applications

Our Abpromise guarantee covers the use of ab85179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
ICC Use a concentration of 5 µg/ml.

Target

  • Tissue specificity

    Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • Sequence similarities

    Contains 2 WAP domains.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q14508 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10406 Human
    • SwissProt: Q14508 Human
    • Unigene: 2719 Human
    • Form

      There are 5 isoforms produced by alternative splicing.
    • Alternative names

      • dJ461P17.6 antibody
      • EDDM4 antibody
      • epididymal protein 4 antibody
      • Epididymal secretory protein E4 antibody
      • Epididymis specific whey acidic protein type four disulfide core antibody
      • HE 4 antibody
      • Major epididymis specific protein E4 antibody
      • Major epididymis-specific protein E4 antibody
      • MGC57529 antibody
      • Putative protease inhibitor WAP5 antibody
      • WAP 5 antibody
      • WAP domain containing protein HE4 antibody
      • WAP domain containing protein HE4 V4 antibody
      • WAP four disulfide core domain 2 antibody
      • WAP four disulfide core domain protein 2 antibody
      • WAP four-disulfide core domain protein 2 antibody
      • WAP5 antibody
      • WFDC 2 antibody
      • WFDC2 antibody
      • WFDC2_HUMAN antibody
      see all

    Images

    • Immunocytochemistry - Anti-HE4 antibody (ab85179)
      Immunocytochemistry - Anti-HE4 antibody (ab85179)

      ab85179 staining WAP four-disulfide core domain protein 2 precursor in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85179 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Western blot - Anti-HE4 antibody (ab85179)
      Western blot - Anti-HE4 antibody (ab85179)
      All lanes : Anti-HE4 antibody (ab85179) at 1 µg/ml

      Lane 1 : Lung (Human) Tissue Lysate
      Lane 2 : Human ovary tissue lysate - total protein (ab30222)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 13 kDa
      Observed band size: 14 kDa
      why is the actual band size different from the predicted?


      Exposure time: 16 minutes


      Human WAP four-disulfide core domain protein 2 precursor (HE4) contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab85179? Please let us know so that we can cite the reference in this datasheet.

    ab85179 has been referenced in 2 publications.

    • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). ICC/IF ; Human . PubMed: 26327314
    • Eade CR  et al. HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria. Protein Pept Lett 22:672-80 (2015). PubMed: 25751267

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HE4 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (15 % Tris-HCl criterion precast gel (Biorad))
    Sample
    Horse Cell lysate - other (equine concentrated oviductal flush)
    Specification
    equine concentrated oviductal flush
    Treatment
    concentrated (centrivap) + resuspended
    Blocking step
    Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: 18°C
    Read More

    Ms. Hilde Nelis

    Verified customer

    Submitted Oct 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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