Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16658] to HE4
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-HE4 antibody [EPR16658]
See all HE4 primary antibodies
DescriptionRabbit monoclonal [EPR16658] to HE4
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Recombinant fragment within Human HE4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q14508
- WB: Human ovary cancer and NIH:OVCAR-3 whole cell lysate. IHC-P: Human ovarian carcinoma. IP: OVCAR-3 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 17-25 kDa (predicted molecular weight: 13 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Tissue specificityExpressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
Sequence similaritiesContains 2 WAP domains.
- Information by UniProt
FormThere are 5 isoforms produced by alternative splicing.
- dJ461P17.6 antibody
- EDDM4 antibody
- epididymal protein 4 antibody
All lanes : Anti-HE4 antibody [EPR16658] (ab200828) at 1/1000 dilution
Lane 1 : Human ovary cancer whole cell lysate
Lane 2 : NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 17-25 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking/dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID:15781627
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling HE4 with ab200828 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on Human ovarian carcinoma tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
HE4 was immunoprecipitated from 1mg of NIH:OVCAR-3 (Human ovary adenocarcinoma ) whole cell lysate with ab200828 at 1/120 dilution.
Western blot was performed from the immunoprecipitate using ab200828 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate 10µg.
Lane 2: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate following IP.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200828 in NIH:OVCAR-3 (human ovary adenocarcinoma) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.
ab200828 has been referenced in 2 publications.
- Wang J et al. Interaction of HE4 and ANXA2 exists in various malignant cells-HE4-ANXA2-MMP2 protein complex promotes cell migration. Cancer Cell Int 19:161 (2019). PubMed: 31210752
- Celik B & Bulut T Human epididymis protein 4 may not be a reliable screening biomarker for detecting lung carcinoma patients. Biomed Rep 7:297-300 (2017). PubMed: 29085624