Overview

  • Product name

    Anti-HE4 antibody [EPR16658]
    See all HE4 primary antibodies
  • Description

    Rabbit monoclonal [EPR16658] to HE4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HE4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14508

  • Positive control

    • WB: Human ovary cancer and NIH:OVCAR-3 whole cell lysate. IHC-P: Human ovarian carcinoma. IP: OVCAR-3 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16658
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 17-25 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/120.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Tissue specificity

      Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
    • Sequence similarities

      Contains 2 WAP domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Form

      There are 5 isoforms produced by alternative splicing.
    • Alternative names

      • dJ461P17.6 antibody
      • EDDM4 antibody
      • epididymal protein 4 antibody
      • Epididymal secretory protein E4 antibody
      • Epididymis specific whey acidic protein type four disulfide core antibody
      • HE 4 antibody
      • Major epididymis specific protein E4 antibody
      • Major epididymis-specific protein E4 antibody
      • MGC57529 antibody
      • Putative protease inhibitor WAP5 antibody
      • WAP 5 antibody
      • WAP domain containing protein HE4 antibody
      • WAP domain containing protein HE4 V4 antibody
      • WAP four disulfide core domain 2 antibody
      • WAP four disulfide core domain protein 2 antibody
      • WAP four-disulfide core domain protein 2 antibody
      • WAP5 antibody
      • WFDC 2 antibody
      • WFDC2 antibody
      • WFDC2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-HE4 antibody [EPR16658] (ab200828) at 1/1000 dilution

      Lane 1 : Human ovary cancer whole cell lysate
      Lane 2 : NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 13 kDa
      Observed band size: 17-25 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

       

      The expression profile observed is consistent with what has been described in the literature PMID:15781627

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling HE4 with ab200828 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on Human ovarian carcinoma tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • HE4 was immunoprecipitated from 1mg of NIH:OVCAR-3 (Human ovary adenocarcinoma ) whole cell lysate with ab200828 at 1/120 dilution.

      Western blot was performed from the immunoprecipitate using ab200828 at 1/1000 dilution.

      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate 10µg.

      Lane 2: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate following IP.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200828 in NIH:OVCAR-3 (human ovary adenocarcinoma) whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.

    References

    This product has been referenced in:

    • Celik B & Bulut T Human epididymis protein 4 may not be a reliable screening biomarker for detecting lung carcinoma patients. Biomed Rep 7:297-300 (2017). Read more (PubMed: 29085624) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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