The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Heat Shock Factor 2 Binding Protein (HSF2BP) associates with Heat Shock Factor 2 (HSF2). The interaction occurs between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation.
ab26149 staining human Heat shock factor 2 binding protein (4.0 - 8.0 ug/ml) in human kidney. Positively stained cells are epithelial cells of the renal tubule (Indicated with arrows). Magnification 400x.