Overview

  • Product name

  • Description

    Rabbit polyclonal to HEATR1
  • Host species

    Rabbit
  • Specificity

    ab200693 detects endogenous levels of HEATR1 protein.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HEATR1 aa 974-1019. The exact sequence is proprietary.
    Sequence:

    IQDLATL FEELQREKKL KSHQKLSETL KNLLSCVYSC PSYIAKDLM


    Database link: Q9H583

  • Positive control

    • HEK293T, sp2/0 and PC12 whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.097% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab200693 was purified by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-HEATR1 antibody (ab200693)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : Sp2/0 whole cell lysate
    Lane 3 : PC12 whole cell lysate

References

ab200693 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - other (glioma cells (GMB))
Gel Running Conditions
Reduced Denaturing (Also tried all other options)
Loading amount
15 µg
Treatment
Also tried with cultures treated with HeatR1 siRNA
Specification
glioma cells (GMB)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: rt°C

Dr. Claudia Barros

Verified customer

Submitted Oct 01 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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