Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesContains 2 HEAT repeats.
- Information by UniProt
- HEAT repeat containing 3 antibody
- HEAT repeat-containing protein 3 antibody
- HEAT3_HUMAN antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab122762 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122762, at 1/1000 dilution, staining HEATR3 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.
ab122762 has not yet been referenced specifically in any publications.