Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesBelongs to the HEATR5 family.
Contains 3 HEAT repeats.
- Information by UniProt
- HEAT repeat-containing protein 5B antibody
- heatr5b antibody
- HTR5B_HUMAN antibody
- KIAA1414 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab126296 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab126296 at 1/200 dilution, staining HEATR5B in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
ab126296 has not yet been referenced specifically in any publications.