Overview

  • Product name
    Anti-HEATR6 antibody
  • Description
    Rabbit polyclonal to HEATR6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 1052-1175 of Human HEATR6.

  • Positive control
    • RT-4 and U-251 MG cell lysates; Human kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (ab122131)
    Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (ab122131)
    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Western blot - Anti-HEATR6 antibody (ab122131)
    Western blot - Anti-HEATR6 antibody (ab122131)
    All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (ab122131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (ab122131)
    ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.

References

ab122131 has not yet been referenced specifically in any publications.

Publishing research using ab122131? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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