Anti-HEATR6 antibody (ab122131)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-HEATR6 antibody -
Description
Rabbit polyclonal to HEATR6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 1052-1175 of Human HEATR6.
-
Positive control
- RT-4 and U-251 MG cell lysates; Human kidney tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab122131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
| WB | 1/250 - 1/500. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Amplification-dependent oncogene. -
Tissue specificity
Amplified in breast cancer cell lines MCF7 and BT474. -
Sequence similarities
Contains 4 HEAT repeats. - Information by UniProt
-
Database links
- Entrez Gene: 63897 Human
- SwissProt: Q6AI08 Human
- Unigene: 591183 Human
-
Alternative names
- Amplified in breast cancer protein 1 antibody
- HEAT repeat-containing protein 6 antibody
- HEAT6_HUMAN antibody
- HEATR6 antibody
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-HEATR6 antibody (ab122131)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122131 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-HEATR6 antibody (ab122131)All lanes : Anti-HEATR6 antibody (ab122131) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEATR6 antibody (ab122131)ab122131, at a 1/200 dilution, staining HEATR6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References
ab122131 has not yet been referenced specifically in any publications.
