Overview

  • Product name
    Anti-heavy chain cardiac Myosin antibody [BA-G5]
    See all heavy chain cardiac Myosin primary antibodies
  • Description
    Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Cow skeletal muscle myosin

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab50967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/100.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20139090

Target

Images

  • Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa

  • ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI.

    See Abreview

  • ab50967 staining mouse heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence.
  • All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • ab50967 staining heavy chain cardiac Myosin in Mouse whole embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Long C  et al. Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing. Sci Adv 4:eaap9004 (2018). Read more (PubMed: 29404407) »
  • Fan ZG  et al. MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Mol Med Rep 17:5658-5665 (2018). Read more (PubMed: 29484401) »
See all 40 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cardiomyocyte)
Permeabilization
Yes - NP40
Specification
Cardiomyocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 21 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 27 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Rat Tissue sections (heart)
Specification
heart
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde

Dr. Shyam Sundar Nandi

Verified customer

Submitted Nov 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse whole embryo)
Specification
mouse whole embryo
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 29 2012

Answer

Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unseren Antikörpern hatten.

Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge zu Veränderung Ihres Protokolls machen:

ab50967

Da Sie diesen Antikörper schon erfolgreich benutz haben, muss ich annehmen, dass Sie dieses Mal leider eine schlechtes Röhrchen bekommen haben. Deshalb wurde ich Ihnen gerne ein Ersatzröhrchen anbieten.


ab63602

1.) Ich schlage vor BSA als Blockierungsreagenz zu verwenden. Viele Antiköper geben bessere Signale mit BSA. Ein Beispiel ist der GAPDH Antikörper ab9385. Bitte folgen Sie diesem Link um deinen Vergleich von Milch und BSA zu sehen:

https://www.abcam.com/index.html?datasheet=9385 (or use the following: https://www.abcam.com/index.html?datasheet=9385).

2.) Außerdem möchte Ich vorschlagen, ab63602 auf einem frischen Blot einzusetzen. Falls nicht viel nNOS vorhanden ist, kann es sein das nach dem Strippen nicht mehr genug Protein auf der Membran ist um detektiert zu werden.

3.) Bei schwachen oder fehlenden Signalen kann das Blockierungagens in der Antikörperlösung weggelassen werden.

4.) Laut dem EST Profile von NCBI unigene, ist nNOS nicht oder kaum im Herzen exprimiert. ich schlage deshalb vor eine Positivkontrolle zu verwenden. Gehirn und Skelettmuskel zeigen eine hohe Expression.


Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte und zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Answer

Thank you for contacting us. Unfortunately, I would not recommend using ab66470 as a positive control in Western blot: It is a synthetic peptide derived from within residues 600 - 700 of Human beta-cardiac myosin heavy chain. This peptide was used as an immunogen for an antibody and is only 20 amino acids in length. Therefore, it is hardly suitable for Western blot. I apologise for the misleading information on our datasheet. Indeed, we do have two anti-MYH7 (heavy chain cardiac Myosin) antibodies available in our catalog: ab15 and ab50967 which are guaranteed to react with myosins with the indicated affinities. All tested applications and species are specified on our datasheets, which are updated as soon as any new information is brought to our attention. Please find more detailed information here: Click here (or use the following: https://www.abcam.com/index.html?datasheet=15). Click here (or use the following: https://www.abcam.com/index.html?datasheet=50967). Although we do not have a suitable purified control protein available, I would suggest one of the following lysates. The above mentioned antibodies have been shown to give positive signals with these tissues. Furthermore, these lysates can be of use in future experiments as a positive control for other proteins than myosin as well. ab3869: Click here (or use the following: https://www.abcam.com/index.html?datasheet=3869). ab30291: Click here (or use the following: https://www.abcam.com/index.html?datasheet=30291). I am pleased to let you know that all our products are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. Furthermore, we do not currently have an image of ab15 in Western blot. Therefore, I can offer a discount off a future purchase if you buy ab15 now, use it Western blot and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody. If you are interested in details of this offer, I would be pleased to send you more information on request. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cells (derived from murine atrial tumor))
Specification
HL-1 cells (derived from murine atrial tumor)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (heart smooth muscle)
Loading amount
30 µg
Specification
heart smooth muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cardiac)
Specification
cardiac
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 05 2010

1-10 of 12 Abreviews or Q&A

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