Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)
- Datasheet
- Specific References (39)
- Protocols
Overview
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Product nameAnti-heavy chain cardiac Myosin antibody [BA-G5]
See all heavy chain cardiac Myosin primary antibodies -
DescriptionMouse monoclonal [BA-G5] to heavy chain cardiac Myosin
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human, Pig
Predicted to work with: Cow
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Immunogen
Cow skeletal muscle myosin
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberBA-G5
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IsotypeIgG2b
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab50967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). | |
| IHC-P | 1/100. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20139090 |
Target
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FunctionMuscle contraction.
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Involvement in diseaseAtrial septal defect 3
Cardiomyopathy, familial hypertrophic 14
Cardiomyopathy, dilated 1EE
Sick sinus syndrome 3 -
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain. -
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
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Database links
- Entrez Gene: 100296004 Cow
- Entrez Gene: 282714 Cow
- Entrez Gene: 4624 Human
- Entrez Gene: 4625 Human
- Entrez Gene: 140781 Mouse
- Entrez Gene: 17888 Mouse
- Entrez Gene: 396860 Pig
- Entrez Gene: 29556 Rat
see all -
Alternative names
- cardiac muscle alpha isoform antibody
- MYH6 antibody
- MYH6_HUMAN antibody
see all
Images
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![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967_1_2.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/heavy-chain-cardiac-Myosin-Primary-antibodies-ab50967-27.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Image courtesy of an anonymous Abreview.ab50967 staining heavy chain cardiac Myosin in murine HL-1 cells (derived from an atrial tumor) by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 10% serum for 30 minutes at 21°C and then incubated with ab50967 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a goat anti-mouse polyclonal conjugated to Alexa-Fluor 488, used at a 1/400 dilution. Counterstained with DAPI. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)This image is courtesy of an Abreview submitted by Antibody Solutions Ltdab50967 staining mouse heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6, prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
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![Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/heavy-chain-cardiac-Myosin-Primary-antibodies-ab50967-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Image from Mayers CM et al, J Biol Chem. 2010 Apr 16;285(16):12344-54. Epub 2010 Feb 5, Fig 3.ab50967 at a 1/200 dilution staining heavy chain cardiac Myosin in murine heterozygote cardiomyocytes by Immunocytochemistry/ Immunofluorescence. -
![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/heavy-chain-cardiac-Myosin-Primary-antibodies-ab50967-2.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/heavy-chain-cardiac-Myosin-Primary-antibodies-ab50967-38.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)This image is courtesy of an anonymous Abreviewab50967 staining heavy chain cardiac Myosin in Mouse whole embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Long C et al. Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing. Sci Adv 4:eaap9004 (2018). Read more (PubMed: 29404407) »
- Fan ZG et al. MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Mol Med Rep 17:5658-5665 (2018). Read more (PubMed: 29484401) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"