Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)
![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62767-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg "Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)")
Key features and details
- Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Overview
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Product name
Anti-heavy chain cardiac Myosin antibody [BA-G5]
See all heavy chain cardiac Myosin primary antibodies -
Description
Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Cow, Human, Pig
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BA-G5 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
Images
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Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa -
![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62765-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (68)
ab50967 has been referenced in 68 publications.
- Mouton AJ et al. Interaction of Obesity and Hypertension on Cardiac Metabolic Remodeling and Survival Following Myocardial Infarction. J Am Heart Assoc 10:e018212 (2021). PubMed: 33666098
- Dai C et al. Lactate Dehydrogenase A Governs Cardiac Hypertrophic Growth in Response to Hemodynamic Stress. Cell Rep 32:108087 (2020). PubMed: 32877669
- Ouyang H et al. Combination of melatonin and irisin ameliorates lipopolysaccharide-induced cardiac dysfunction through suppressing the Mst1-JNK pathways. J Cell Physiol 235:6647-6659 (2020). PubMed: 31976559
- Mohammed SA et al. Allylmethylsulfide, a Sulfur Compound Derived from Garlic, Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats. Oxid Med Cell Longev 2020:7856318 (2020). PubMed: 32617142
- Heimerl M et al. Neuraminidase-1 promotes heart failure after ischemia/reperfusion injury by affecting cardiomyocytes and invading monocytes/macrophages. Basic Res Cardiol 115:62 (2020). PubMed: 32975669