Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)
![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62767-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg "Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)")
Key features and details
- Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Overview
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Product name
Anti-heavy chain cardiac Myosin antibody [BA-G5]
See all heavy chain cardiac Myosin primary antibodies -
Description
Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Tissue, cells or virus corresponding to Cow heavy chain cardiac Myosin.
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
BA-G5 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Rat
|
| All applications |
Rabbit
Cow
Human
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
Images
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Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa -
![Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)](https://www.abcam.com/ps/products/50/ab50967/Images/ab50967-62765-anti-heavy-chain-cardiac-myosin-antibody-ba-g5-western-blot.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)All lanes : Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 48 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
References (60)
ab50967 has been referenced in 60 publications.
- Dai C et al. Lactate Dehydrogenase A Governs Cardiac Hypertrophic Growth in Response to Hemodynamic Stress. Cell Rep 32:108087 (2020). PubMed: 32877669
- Yi M et al. Ion Therapy: A Novel Strategy for Acute Myocardial Infarction. Adv Sci (Weinh) 6:1801260 (2019). PubMed: 30643722
- Liu M et al. LncRNA-MEG3 promotes bovine myoblast differentiation by sponging miR-135. J Cell Physiol N/A:N/A (2019). PubMed: 30887511
- Shang L et al. Plantamajoside attenuates isoproterenol-induced cardiac hypertrophy associated with the HDAC2 and AKT/ GSK-3ß signaling pathway. Chem Biol Interact 307:21-28 (2019). PubMed: 31009642
- Sun Q et al. SEMA6D regulates perinatal cardiomyocyte proliferation and maturation in mice. Dev Biol 452:1-7 (2019). PubMed: 31042497
