Western blot abreview for Anti-MYH6 antibody [BA-G5]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (heart smooth muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
heart smooth muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 223 kDa

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Submitted Sep 16 2011

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