Immunocytochemistry/ Immunofluorescence abreview for Anti-MYH6 antibody [BA-G5]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cells (derived from murine atrial tumor))
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HL-1 cells (derived from murine atrial tumor)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 1% goat serum + 0.1% Triton X-100
Dilution
1/200

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-mouse IgG Alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Image: confocal microscopy image of HL-1 cell stained with BA-G5 anti-MHC primary antibody and anti-mouse-alexa488 secondary antibody (green) nucleus stained with DAPI (blue).

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Submitted Sep 23 2011

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