Overview

  • Product name

    Anti-heavy chain Myosin/MYH3 antibody
  • Description

    Rabbit polyclonal to heavy chain Myosin/MYH3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human heavy chain Myosin/MYH3 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab124298)

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates.
  • General notes

     This product was previously labelled as heavy chain Myosin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function

    Muscle contraction.
  • Involvement in disease

    Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
    Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Developmental stage

    Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links

  • Alternative names

    • embryonic antibody
    • fast skeletal muscle antibody
    • HEMHC antibody
    • Muscle embryonic myosin heavy chain 3 antibody
    • Muscle embryonic myosin heavy chain antibody
    • MYH 3 antibody
    • Myh3 antibody
    • MYH3_HUMAN antibody
    • MYHC EMB antibody
    • MYHSE 1 antibody
    • MYHSE1 antibody
    • Myosin heavy chain 3 antibody
    • Myosin heavy chain 3 skeletal muscle embryonic antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain fast skeletal muscle embryonic antibody
    • Myosin Heavy Polypeptide 3 antibody
    • Myosin heavy polypeptide 3 skeletal muscle embryonic antibody
    • Myosin skeletal heavy chain embryonic 1 antibody
    • Myosin-3 antibody
    • SMHCE antibody
    see all

Images

  • All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • IHC image of Fast Myosin Skeletal Heavy chain staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124205, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Heart (Human) Tissue Lysate - fetal normal tissue
    Lane 6 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 223 kDa
    Observed band size: 223 kDa
    Additional bands at: 190 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References

This product has been referenced in:

  • Wang F  et al. The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells. BMC Cancer 18:360 (2018). Read more (PubMed: 29609556) »
  • Asai R  et al. Amniogenic somatopleure: a novel origin of multiple cell lineages contributing to the cardiovascular system. Sci Rep 7:8955 (2017). Read more (PubMed: 28827655) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 09 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 03 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 09 2014

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