Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Belongs to the NDC80/HEC1 family.
Expression peaks in mitosis.
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
All lanes : Anti-HEC1 antibody (ab97610) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Lane 1: 3µg ab97610 were used to precipitate 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab97610 at 1µg/ml. Lane 2: same, using a control IgG. Detection: chemiluminescence with exposure time of 10 seconds.