Overview

  • Product name

  • Description

    Goat polyclonal to HEC1/HEC
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HEC1/HEC aa 630-642 (C terminal) (Cysteine residue).
    Sequence:

    YEKKATLIKSSEE


    Database link: NP_006092.1

  • Positive control

    • Human adrenal and liver tissues; HeLa cell lysate.
  • General notes

     This product was previously labelled as HEC1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab117415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 74 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similarities

    Belongs to the NDC80/HEC1 family.
  • Developmental stage

    Expression peaks in mitosis.
  • Post-translational
    modifications

    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links

  • Alternative names

    • from Entrez Gene ; antibody
    • HEC antibody
    • Highly expressed in cancer antibody
    • Highly expressed in cancer protein antibody
    • Highly expressed in cancer rich in leucine heptad repeats antibody
    • HsHec1 antibody
    • hsNDC80 antibody
    • Kinetochore associated 2 antibody
    • Kinetochore associated protein 2 antibody
    • Kinetochore protein Hec1 antibody
    • Kinetochore protein NDC80 homolog antibody
    • Kinetochore-associated protein 2 antibody
    • KNTC2 antibody
    • ndc80 antibody
    • NDC80 homolog kinetochore complex component antibody
    • NDC80 kinetochore complex component homolog antibody
    • NDC80, S. cerevisiae, homolog of antibody
    • NDC80_HUMAN antibody
    • Retinoblastoma associated protein HEC antibody
    • Retinoblastoma-associated protein HEC antibody
    • TID3 antibody
    see all

Images

  • ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Anti-HEC1/HEC antibody (ab117415) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 74 kDa

References

ab117415 has not yet been referenced specifically in any publications.

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