Key features and details
- Rabbit polyclonal to HEC1/HEC
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HEC1/HEC antibody
See all HEC1/HEC primary antibodies
DescriptionRabbit polyclonal to HEC1/HEC
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human HEC1/HEC aa 351-400. The exact sequence is proprietary.
Database link: O14777
- IHC-P: Human testis tissue. ICC/IF: HUVEC cells. WB: Jurkat cell extract.
This product was previously labelled as HEC1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 74 kDa.|
FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Sequence similaritiesBelongs to the NDC80/HEC1 family.
Developmental stageExpression peaks in mitosis.
modificationsPhosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
HUVEC (Human umbilical vein endothelial cell line) cells labelling HEC1/HEC using ab230916 (green) at 1/100 dilution in ICC/IF. The picture on the right was treated with the synthesized peptide.
Formalin-fixed, paraffin-embedded human testis tissue tissue stained for HEC1/HEC using ab230916 at 1/50 dilution in immunohstochemical analysis.
All lanes : Anti-HEC1/HEC antibody (ab230916) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 2 : Jurkat cell extract with immunizing peptide
Predicted band size: 74 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230916 has not yet been referenced specifically in any publications.