This antibody was tested at 1:100, 1:200 and 1:500 dilutions for both 1hr and overnight incubations. It does not appear to recognize kinetochore associated HEC1 under these conditions as no focal patterns were observed in mitotic cells.
Thank you for your valuable feedback! ICC/IF was not a tested application for ab3393 and, based on your data we will recommend that researchers interested in detecting HEC1 by ICC/IF look for a diferent antibody!
Dr. Kirk Mcmanus
Submitted Dec 15 2006
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