Immunocytochemistry/ Immunofluorescence abreview for Anti-HEC1/HEC antibody

Poor
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Goat FITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein

Additional data

Additional Notes
This antibody was tested at 1:100, 1:200 and 1:500 dilutions for both 1hr and overnight incubations. It does not appear to recognize kinetochore associated HEC1 under these conditions as no focal patterns were observed in mitotic cells.
Abcam response
Thank you for your valuable feedback! ICC/IF was not a tested application for ab3393 and, based on your data we will recommend that researchers interested in detecting HEC1 by ICC/IF look for a diferent antibody!

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 15 2006

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