Overview

  • Product name

    Anti-HEC1/HEC (phospho S55) antibody
    See all HEC1/HEC primary antibodies
  • Description

    Rabbit polyclonal to HEC1/HEC (phospho S55)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HEC1/HEC (phospho S55) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O14777

  • Positive control

    • WB: HeLa treated with Nocodazole, whole cell extract.
  • General notes

     This product was previously labelled as HEC1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.

Target

  • Function

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similarities

    Belongs to the NDC80/HEC1 family.
  • Developmental stage

    Expression peaks in mitosis.
  • Post-translational
    modifications

    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • Database links

  • Alternative names

    • from Entrez Gene ; antibody
    • HEC antibody
    • Highly expressed in cancer antibody
    • Highly expressed in cancer protein antibody
    • Highly expressed in cancer rich in leucine heptad repeats antibody
    • HsHec1 antibody
    • hsNDC80 antibody
    • Kinetochore associated 2 antibody
    • Kinetochore associated protein 2 antibody
    • Kinetochore protein Hec1 antibody
    • Kinetochore protein NDC80 homolog antibody
    • Kinetochore-associated protein 2 antibody
    • KNTC2 antibody
    • ndc80 antibody
    • NDC80 homolog kinetochore complex component antibody
    • NDC80 kinetochore complex component homolog antibody
    • NDC80, S. cerevisiae, homolog of antibody
    • NDC80_HUMAN antibody
    • Retinoblastoma associated protein HEC antibody
    • Retinoblastoma-associated protein HEC antibody
    • TID3 antibody
    see all

Images

  • All lanes : Anti-HEC1/HEC (phospho S55) antibody (ab229724) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml Nocodazole for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 74 kDa



    7.5% SDS-PAGE gel.

References

ab229724 has not yet been referenced specifically in any publications.

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