Key features and details
- Rabbit polyclonal to HEC1/HEC (phospho S55)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HEC1/HEC (phospho S55) antibody
See all HEC1/HEC primary antibodies
DescriptionRabbit polyclonal to HEC1/HEC (phospho S55)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HEC1/HEC (phospho S55) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O14777
- WB: HeLa treated with Nocodazole, whole cell extract.
This product was previously labelled as HEC1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 74 kDa.|
FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Sequence similaritiesBelongs to the NDC80/HEC1 family.
Developmental stageExpression peaks in mitosis.
modificationsPhosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
- Information by UniProt
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
All lanes : Anti-HEC1/HEC (phospho S55) antibody (ab229724) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml Nocodazole for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229724 has not yet been referenced specifically in any publications.