• Product name

  • Description

    Rabbit polyclonal to HECTD1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 1-50 of Human HECTD1 (AAP13073.1).

  • Positive control

    • HeLa and 293T whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.



Our Abpromise guarantee covers the use of ab101992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 290 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. May be required for development of the head mesenchyme and neural tube closure.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the UPL family. K-HECT subfamily.
    Contains 4 ANK repeats.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 MIB/HERC2 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ligase for inhibin receptor antibody
    • E3 ubiquitin protein ligase HECTD1 antibody
    • E3 ubiquitin-protein ligase HECTD1 antibody
    • EULIR antibody
    • HECD1_HUMAN antibody
    • HECT domain containing protein 1 antibody
    • HECT domain-containing protein 1 antibody
    • HECTD1 antibody
    • KIAA1131 antibody
    see all


  • All lanes : Anti-HECTD1 antibody (ab101992) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 290 kDa

    Exposure time: 3 minutes
  • ab101992 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101992 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes


This product has been referenced in:

  • Saez I  et al. Insights into the ubiquitin-proteasome system of human embryonic stem cells. Sci Rep 8:4092 (2018). Read more (PubMed: 29511261) »
  • Flack JE  et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). Read more (PubMed: 28689657) »
See all 4 Publications for this product

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