Anti-HECTD2 antibody [EPR11554] (ab173572)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-HECTD2 antibody [EPR11554]
See all HECTD2 primary antibodies -
DescriptionRabbit monoclonal [EPR11554] to HECTD2
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human HECTD2 aa 600-700 (Cysteine residue). The exact sequence is proprietary.
Database link: Q5U5R9 -
Positive control
- MCF-7, NIH:OVCAR-3 and HeLa cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11554
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab173572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 88 kDa. |
Target
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FunctionProbable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
- Information by UniProt
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Database links
- Entrez Gene: 143279 Human
- Entrez Gene: 226098 Mouse
- Entrez Gene: 100172010 Orangutan
- Entrez Gene: 309514 Rat
- SwissProt: Q5U5R9 Human
- SwissProt: Q8CDU6 Mouse
- SwissProt: Q5RD78 Orangutan
- Unigene: 596096 Human
see all -
Alternative names
- FLJ16050 antibody
- FLJ37306 antibody
- HECD2_HUMAN antibody
see all
Images
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![Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572)](https://www.abcam.com/ps/products/173/ab173572/Images/ab173572-197088-ab1735721.jpg)
Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572)All lanes : Anti-HECTD2 antibody [EPR11554] (ab173572) at 1/1000 dilution
Lane 1 : MCF-7 cell lysates
Lane 2 : NIH:OVCAR-3 cell lysates
Lane 3 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 88 kDa
Datasheets and documents
Protocols
References
ab173572 has not yet been referenced specifically in any publications.
